VvSWEET7 Is a Mono- and Disaccharide Transporter Up-Regulated in Response to Botrytis cinerea Infection in Grape Berries
VvSWEET7 Is a Mono- and Disaccharide Transporter Up-Regulated in Response to Botrytis cinerea Infection in Grape Berries
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DOI：
10.3389/fpls.2019.01753
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发表时间：
2020-01-27
影响因子：
5.6
通讯作者：
Geros, Hernani
中科院分区：
文献类型：
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作者：
Breia, Richard;Conde, Artur;Geros, Hernani
新发现的SWEETROSINS是一种高容量、低亲和力的糖转运蛋白，在糖外排至关重要的许多生理机制中发挥重要作用。SWEET是病原体操纵的理想靶标，并且它们的表达可以在感染期间转录重编程。到目前为止，很少有植物甜转运蛋白的功能，特别是在葡萄藤。在这项研究中，在葡萄孢菌易感品种“Trincadeira”中，我们彻底分析了灰葡萄孢菌感染葡萄浆果中关键SWEET基因的基因表达谱的修饰。VvSWEET 7和VvSWEET 15可能在果实发育和葡萄孢属感染期间起重要作用，因为它们分别在绿色和成熟阶段强烈表达，并且响应于感染而明显上调。还有B灰霉病菌感染下调了绿色期VvSWEET 17 a的表达，下调了变熟期VvSWEET 10和VvSWEET 17 d的表达，下调了成熟期VvSWEET 11的表达。VvSWEET 7的功能特征在于在酿酒酵母中作为低亲和力、高容量的葡萄糖和蔗糖转运蛋白的异源表达，其对葡萄糖的K（m）为15.42 mM，对蔗糖的K（m）为40.08 mM。VvSWEET 7-GFP和VvSWEET 15-GFP融合蛋白在本氏烟草表皮细胞中瞬时表达，共聚焦显微镜可以观察到这两种蛋白质明显定位于质膜。总之，VvSWEET转运蛋白是葡萄浆果发育过程中糖动员的重要参与者，它们的表达响应于葡萄孢菌感染而转录重编程。
The newly-identified SWEETs are high-capacity, low-affinity sugar transporters with important roles in numerous physiological mechanisms where sugar efflux is critical. SWEETs are desirable targets for manipulation by pathogens and their expression may be transcriptionally reprogrammed during infection. So far, few plant SWEET transporters have been functionally characterized, especially in grapevine. In this study, in the Botrytis-susceptible variety "Trincadeira," we thoroughly analyzed modifications in the gene expression profile of key SWEET genes in Botrytis cinerea-infected grape berries. VvSWEET7 and VvSWEET15 are likely to play an important role during fruit development and Botrytis infection as they are strongly expressed at the green and mature stage, respectively, and were clearly up-regulated in response to infection. Also, B. cinerea infection down-regulated VvSWEET17a expression at the green stage, VvSWEET10 and VvSWEET17d expression at the veraison stage, and VvSWEET11 expression at the mature stage. VvSWEET7 was functionally characterized by heterologous expression in Saccharomyces cerevisiae as a low-affinity, high-capacity glucose and sucrose transporter with a K (m) of 15.42 mM for glucose and a K (m) of 40.08 mM for sucrose. VvSWEET7-GFP and VvSWEET15-GFP fusion proteins were transiently expressed in Nicotiana benthamiana epidermal cells and confocal microscopy allowed to observe that both proteins clearly localize to the plasma membrane. In sum, VvSWEETs transporters are important players in sugar mobilization during grape berry development and their expression is transcriptionally reprogrammed in response to Botrytis infection.