Production of plant-specific tyramine derivatives by dual expression of tyramine N-hydroxycinnamoyltransferase and 4-coumarate:coenzyme A ligase in Escherichia coli
Production of plant-specific tyramine derivatives by dual expression of tyramine N-hydroxycinnamoyltransferase and 4-coumarate:coenzyme A ligase in Escherichia coli
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DOI：
10.1007/s10529-009-0032-3
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发表时间：
2009-09-01
影响因子：
2.7
通讯作者：
Back, Kyoungwhan
中科院分区：
文献类型：
--
作者：
Kang, Kiyoon;Park, Munyoung;Back, Kyoungwhan
利用从拟南芥和辣椒中分别克隆的4-香豆酸辅酶A连接酶和酪胺n -羟基肉桂酰基转移酶（THT）基因，在大肠杆菌中同时制备了阿铁酰乙胺（FT）、4-香豆酰乙胺（CT）和咖啡酰乙胺（CaT）等植物特异性生物活性化合物。在重组大肠杆菌中同时添加底物，导致多种酪胺衍生物高产地分泌到培养基中：CT (189 mg l(-1)), FT (135 mg l(-1)), CaT （40 mg l(-1)）。此外，重组大肠杆菌也产生了一系列多巴胺衍生物，如阿魏酰多巴胺，尽管浓度很低，这是由于THT能够接受多巴胺作为底物。
Plant-specific bioactive compounds including feruloyltyramine (FT), 4-coumaroyltyramine (CT), and caffeoyltyramine (CaT) were simultaneously produced in Escherichia coli by heterologous expression of two biosynthetic genes encoding 4-coumarate:coenzyme A ligase and tyramine N-hydroxycinnamoyltransferase (THT) cloned from Arabidopsis thaliana and pepper, respectively. Simultaneous supplementation of substrates to the recombinant E. coli resulted in the secretion of multiple tyramine derivatives into the medium at high yield: CT (189 mg l(-1)), FT (135 mg l(-1)), CaT (40 mg l(-1)). In addition, the recombinant E. coli also produced, albeit at low concentration, a range of dopamine derivatives such as feruloyldopamine due to THT's ability to accept dopamine as a substrate.