Microarrayed allergens for IgE profiling
Microarrayed allergens for IgE profiling
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DOI：
10.1016/j.ymeth.2003.08.018
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发表时间：
2004-03-01
期刊：
影响因子：
4.8
通讯作者：
Spitzauer, S
中科院分区：
文献类型：
--
作者：
Deinhofer, K;Sevcik, H;Spitzauer, S
I型变态反应的诊断基于既往史、激发试验和总IgE和特异性IgE的血清学测定。目前，体内和体外诊断测试使用从各种过敏原来源（例如，花粉、螨、动物皮屑、霉菌、食物、毒液等）。重组DNA技术在过敏原鉴定领域的应用已经揭示了最常见过敏原的分子本质。迄今为止，不断增加的过敏原序列的数量已经成为可用的和重组过敏原的面板-组装天然过敏原来源的表位复杂性-可以生产。使用重组过敏原而不是天然粗提物进行过敏诊断，使我们能够确定每个患者的个体IgE反应性特征。为了能够全面分析患者的IgE结合模式，以大量的个别过敏原，一种新型的血清学测试是必要的。本文应用基因芯片技术，建立了一种基于基因芯片的多变应原检测系统。(C)2003年爱思唯尔公司All rights reserved.
Diagnosis of type I allergy is based on anamnesis, provocation testing, and serological determination of total and specific IgE. Currently, in vivo and in vitro diagnostic tests employ allergen extracts prepared from various allergen sources (e.g., pollen, mites, animal dander, moulds, foods, venoms, etc.). The application of recombinant DNA technology to the field of allergen characterization has allowed to reveal the molecular nature of the most common allergens. To date a continuously increasing number of allergen sequences has become available and panels of recombinant allergens-assembling the epitope complexity of natural allergens sources-can be produced. The use of recombinant allergens instead of crude, natural extracts for allergy diagnosis allows us to determine the individual IgE reactivity profile of each patient. To enable a comprehensive analysis of the patient's IgE binding pattern to a large number of individual allergens, a new type of serological test is required. In this paper, we applied microarray technology to create a multi-allergen test system, based on microarrayed recombinant allergens. (C) 2003 Elsevier Inc. All rights reserved.