Single delayed rectifier channels in frog atrial cells. Effects of beta-adrenergic stimulation.
Single delayed rectifier channels in frog atrial cells. Effects of beta-adrenergic stimulation.
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青蛙心房细胞中的单个延迟整流通道。
DOI：
10.1016/s0006-3495(90)82610-2
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发表时间：
1990
影响因子：
3.4
通讯作者：
Hartzell,HC
中科院分区：
文献类型：
--
作者：
Duchatelle-Gourdon,I;Hartzell,HC
膜片钳技术与两个移液管用于记录单延迟K+通道（细胞附着电极）和控制的电位和细胞内室的组成（全细胞电极）。在细胞中有30 μ M cAMP和贴片内外的生理钾浓度的情况下，表征了携带外向电流的通道。其开放概率非常低，在对照条件下，仅在5%的斑块中记录到通道。增加细胞内cAMP使在贴片中找到通道的概率增加10倍。该通道具有延迟整流器通道的预期特性。其总体平均值的时间过程类似于同一细胞中的全细胞电流。的电流-电压关系表现出内向整流，与20 pS的斜率电导的线性部分和接近EK的反转电位。开放和关闭的时间分布描述的两个指数的总和，提出了一个复杂的门控方案，涉及两个封闭的状态和两个开放的状态。β-肾上腺素能刺激不改变通道的电导，但增加其开放的可能性。
The patch-clamp technique with two pipettes was used to record single delayed K+ channels (cell-attached electrode) and to control the potential and the composition of the intracellular compartment (whole-cell electrode). With 30 microM cAMP in the cell and physiological potassium concentrations inside and outside the patch, a channel carrying an outward current was characterized. Its open probability was very low and the channel was recorded in only 5% of patches under control conditions. Increasing intracellular cAMP increased the probability of finding a channel in a patch 10-fold. The channel had the characteristics expected of a delayed rectifier channel. The time-course of its ensemble average resembled the whole-cell current in the same cell. The current-voltage relationship exhibited inward rectification, with a slope conductance of 20 pS in the linear portion and a reversal potential close to EK. Both the open- and the closed-time distributions were described by the sum of two exponentials, suggesting a complicated gating scheme involving two closed states and two open states. The beta-adrenergic stimulation did not change the conductance of the channel, but increased its probability of opening.
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青蛙骨骼肌肌膜形成的囊泡中单一钾通道的特性。
DOI：
10.1113/jphysiol.1985.sp015749
发表时间：
1985
期刊：
The Journal of Physiology
影响因子：
--
作者：
N. Standen;P. Stanfield;T. A. Ward
通讯作者：
T. A. Ward
影响因子：
3.4
作者：
CLAPHAM, DE;DEFELICE, LJ
通讯作者：
DEFELICE, LJ
影响因子：
3.4
作者：
BENNETT, P;MCKINNEY, L;KASS, RS
通讯作者：
KASS, RS
心脏浦肯野纤维延迟整流 K 通道的非平稳波动分析。
影响因子：
3.4
作者：
Bennett,PB;Kass,R;Begenisich,T
通讯作者：
Begenisich,T
兔心脏 A-V 结中的慢电流系统
影响因子：
64.8
作者：
A. Noma;H. Irisawa;S. Kokobun;H. Kotake;M. Nishimura;Y. Watanabe
通讯作者：
Y. Watanabe