Feline coronavirus serotypes 1 and 2: Seroprevalence and association with disease in Switzerland
Feline coronavirus serotypes 1 and 2: Seroprevalence and association with disease in Switzerland
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DOI：
10.1128/cdli.12.10.1209-1215.2005
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发表时间：
2005-10-01
期刊：
影响因子：
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通讯作者：
Lutz, H
中科院分区：
文献类型：
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作者：
Kummrow, M;Meli, ML;Lutz, H
为了确定瑞士猫冠状病毒（FCoV）血清型1和2的抗体流行情况及其与不同疾病表现的关系，使用传染性胃肠炎病毒（TGEV）、FCoV 1型和FCoV 2型作为抗原，对瑞士野猫进行了基于免疫荧光试验的血清学研究。对在不同研究背景下从自然感染猫中采集的共计639份血清样本进行了检测。目前的研究表明，FCoV血清型1是瑞士最流行的血清型，其明显流行率为83%。FCoV 1型病毒诱导的抗体滴度高于FCoV 2型，并且更常与临床体征和/或猫传染性腹膜炎相关。在7只实验性感染FCoV I型的猫中的抗体发展显示，随着感染持续时间的进展，FCoV 1型的抗体显著增加，超过FCoV 2型的抗体。抗TGEV、FCoV 1和FCoV 2的抗体滴度与TGEV抗原检测到的血清阳性猫比例最高之间存在显著相关性。我们的结论是，针对FCoV的疫苗应基于FCoV 1型相关抗原，FCoV感染的血清学诊断应使用TGEV，以达到最高的诊断效率。当除了临床体征、血液学和临床化学结果之外还使用血清学作为辅助诊断临床FIP时，TGEV显示出与FCoV抗原相等的诊断效率。
To determine the prevalence of antibodies to feline coronavirus (FCoV) serotypes 1 and 2 in Switzerland and their association with different disease manifestations, a serological study based on immunofluorescence tests was conducted with Swiss field cats using transmissible gastroenteritis virus (TGEV), FCoV type 1 and FCoV type 2 as antigens. A total of 639 serum samples collected in the context of different studies from naturally infected cats were tested. The current study revealed that, with an apparent prevalence of 83%, FCoV serotype 1 is the most prevalent serotype in Switzerland. FCoV type 1 viruses induced higher antibody titers than FCoV type 2, and were more frequently associated with clinical signs and/or feline infectious peritonitis. The antibody development in seven cats experimentally infected with FCoV type I revealed that, with progressing duration of infection, antibodies to FCoV type 1 significantly increased over those to FCoV type 2. There was a significant relationship between antibody titers against TGEV, FCoV 1, and FCoV 2 and TGEV antigen detected the highest proportion of seropositive cats. We conclude that a vaccine against FCoV should be based on FCoV type 1-related antigens and that for serodiagnosis of FCoV infection TGEV should be used to attain the highest diagnostic efficiency. When serology is used in addition to clinical signs, hematology, and clinical chemistry results as an aid to diagnose clinical FIP, TGEV shows a diagnostic efficiency equal to that of a FCoV antigen.