Transcriptomic Analysis of Long Non-coding RNA-MicroRNA-mRNA Interactions in the Nucleus Accumbens Related to Morphine Addiction in Mice.
Transcriptomic Analysis of Long Non-coding RNA-MicroRNA-mRNA Interactions in the Nucleus Accumbens Related to Morphine Addiction in Mice.
复制标题
与小鼠吗啡成瘾相关的伏核中长非编码 RNA-MicroRNA-mRNA 相互作用的转录组分析
DOI：
10.3389/fpsyt.2022.915398
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发表时间：
2022
影响因子：
4.7
通讯作者：
Ma, Chunling
中科院分区：
文献类型：
--
作者：
Li, Xiaojie;Xie, Bing;Lu, Yun;Yang, Hongyu;Wang, Jian;Yu, Feng;Zhang, Ludi;Cong, Bin;Wen, Di;Ma, Chunling
近年来的研究表明，一些非编码RNA（ncRNA）在神经系统发育和神经系统疾病中是染色质动力学和基因表达的重要调节因子。然而，作为竞争性内源性RNA（ceRNA）的长链非编码RNA（lncRNA）在吗啡成瘾中的分子机制仍不清楚。本研究采用RNA测序技术（RNA-seq）检测了吗啡和生理盐水条件位置偏爱（CPP）训练小鼠的丘脑核（NAc）组织中lncRNA、miRNAs和mRNAs的表达谱，发现31个lncRNA、393个miRNAs、共发现371个mRNA，建立了9个差异表达lncRNA相互作用的ceRNA网络基于lncRNA和mRNA共有的预测的miRNA，对10种差异表达的miRNA（DEmiRNA）和12种差异表达的mRNA（DEmRNA）进行比较。进行KEGG途径富集分析以探索DEmRNAs在ceRNA网络中与lncRNA相互作用的潜在功能。这些DEmRNA在突触可塑性相关的通路中富集，包括嘧啶代谢、ECM-受体相互作用和粘着斑。分析lncRNA、miRNA和mRNA的相对表达之间的相关性以进一步验证预测的ceRNA网络，并确定Lnc 15 qD 3-miR-139- 3 p-Lrp 2 ceRNA调控相互作用。这些结果表明，综合网络代表了对lncRNA介导的ceRNA调节机制的新见解，并为吗啡成瘾提供了新的潜在诊断和预后生物标志物。
Recent research suggest that some non-coding RNAs (ncRNAs) are important regulators of chromatin dynamics and gene expression in nervous system development and neurological diseases. Nevertheless, the molecular mechanisms of long non-coding RNAs (lncRNAs), acting as competing endogenous RNAs (ceRNAs), underlying morphine addiction are still unknown. In this research, RNA sequencing (RNA-seq) was used to examine the expression profiles of lncRNAs, miRNAs and mRNAs on the nucleus accumbens (NAc) tissues of mice trained with morphine or saline conditioned place preference (CPP), with differential expression of 31 lncRNAs, 393 miRNAs, and 371 mRNAs found. A ceRNA network was established for reciprocal interactions for 9 differentially expressed lncRNAs (DElncRNAs), 10 differentially expressed miRNAs (DEmiRNAs) and 12 differentially expressed mRNAs (DEmRNAs) based on predicted miRNAs shared by lncRNAs and mRNAs. KEGG pathway enrichment analyses were conducted to explore the potential functions of DEmRNAs interacting with lncRNAs in the ceRNA network. These DEmRNAs were enriched in synaptic plasticity-related pathways, including pyrimidine metabolism, ECM-receptor interaction, and focal adhesion. The correlation between the relative expression of lncRNAs, miRNAs and mRNAs was analyzed to further validate predicted ceRNA networks, and the Lnc15qD3-miR-139-3p-Lrp2 ceRNA regulatory interaction was determined. These results suggest that the comprehensive network represents a new insight into the lncRNA-mediated ceRNA regulatory mechanisms underlying morphine addiction and provide new potential diagnostic and prognostic biomarkers for morphine addiction.
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影响因子：
4.8
作者：
Gomes JR;Lobo A;Nogueira R;Terceiro AF;Costelha S;Lopes IM;Magalhães A;Summavielle T;Saraiva MJ
通讯作者：
Saraiva MJ
DOI：
10.1016/j.bbrc.2021.12.034
发表时间：
2021-12-20
影响因子：
3.1
作者：
Guo, Ningning;Zhang, Le;Bai, Jie
通讯作者：
Bai, Jie
影响因子：
64.8
作者：
Golden SA;Heshmati M;Flanigan M;Christoffel DJ;Guise K;Pfau ML;Aleyasin H;Menard C;Zhang H;Hodes GE;Bregman D;Khibnik L;Tai J;Rebusi N;Krawitz B;Chaudhury D;Walsh JJ;Han MH;Shapiro ML;Russo SJ
通讯作者：
Russo SJ
影响因子：
11
作者：
Forget, Benoit;Garcia, Elena Martin;Godino, Arthur;Rodriguez, Laura Domingo;Kappes, Vincent;Poirier, Pierre;Andrianarivelo, Andry;Marchan, Eric Senabre;Allichon, Marie-Charlotte;Marias, Melanie;Vanhoutte, Peter;Girault, Jean-Antoine;Maldonado, Rafael;Caboche, Jocelyne
通讯作者：
Caboche, Jocelyne
TFAP2C 激活的 MALAT1 调节多西他赛耐药肺腺癌细胞的化疗耐药性
DOI：
10.1016/j.omtn.2019.01.005
发表时间：
2019-03-01
期刊：
MOLECULAR THERAPY NUCLEIC ACIDS
影响因子：
--
作者：
Chen, Jing;Liu, Xiaobei;Chen, Longbang
通讯作者：
Chen, Longbang