Expression of rck/p54, a DEAD‐box RNA helicase, in gametogenesis and early embryogenesis of mice
Expression of rck/p54, a DEAD‐box RNA helicase, in gametogenesis and early embryogenesis of mice
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DOI：
10.1002/dvdy.20429
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发表时间：
2005-07
影响因子：
2.5
通讯作者：
Kenji Matsumoto;O. Kwon;Hyungtae Kim;Y. Akao
中科院分区：
文献类型：
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作者：
Kenji Matsumoto;O. Kwon;Hyungtae Kim;Y. Akao
rck/p54是一种具有ATP依赖性RNA解旋活性的DEAD‐box RNA解旋酶蛋白。它的直系同源物是酵母有性生殖和秀丽隐杆线虫卵母细胞存活和精子生育所必需的。本研究探讨rck/p54在小鼠配子发生和早期胚胎发生中的表达。westernblot分析表明，rck/p54在卵巢和睾丸中均有高表达。在卵巢中，成熟的卵母细胞在细胞质中强烈表达rck/p54。相反，在睾丸中，精原细胞和初级精母细胞在其细胞质中高表达rck/p54，而在精子细胞中表达减少。有趣的是，rck/p54集中在精子的头部，然后随着这些细胞沿着附睾管成熟，其表达逐渐下降。受精后，rck/p54蛋白及其mRNA在原核期仍有表达，但在桑椹胚和囊胚中的表达水平略有下降。将基于CMV的rck/p54表达载体注射到受精卵的原核中导致早期胚胎发生延迟。RCK转基因小鼠的出生率明显低于其他转基因小鼠。这些结果表明rck/p54可能在哺乳动物配子发生和早期胚胎发生中起重要作用。发展动力学233：1149-1156，2005年。© 2005 Wiley利斯公司
rck/p54 is a DEAD‐box RNA helicase protein with ATP‐dependent RNA‐unwinding activity. Its ortholog is required for sexual reproduction in yeast and for oocyte survival and sperm fertility in Caenorhabditis elegans. In the current study, we investigated the expression of rck/p54 in mouse gametogenesis and early embryogenesis. Western blot analysis revealed that rck/p54 was highly expressed in both the ovary and testis. In the ovary, maturing oocytes strongly expressed rck/p54 in their cytoplasm. In contrast, in the testis, spermatogonia and primary spermatocytes highly expressed rck/p54 in their cytoplasm, but its expression decreased in the spermatids. Interestingly, rck/p54 was concentrated in the heads of spermatozoa; and then its expression gradually decreased as these cells matured along the epididymal duct. After fertilization, rck/p54 protein and its mRNA remained present in the pronucleus phase; and then their expression levels slightly but definitely decreased in morulae and blastocytes. The injection of a CMV‐based rck/p54 expression vector into the pronuclei of fertilized eggs caused a delay in early embryogenesis. In generating RCK transgenic mice, the birth rate of the mice was significantly lower than those of other gene transgenic mice. These findings indicate that rck/p54 may play an important role in gametogenesis and early embryogenesis in mammals. Developmental Dynamics 233:1149–1156, 2005. © 2005 Wiley‐Liss, Inc.