Cisplatin-based DNA sensing with enhanced current response.
Cisplatin-based DNA sensing with enhanced current response.
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DOI：
10.1039/b906734e
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发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Y. Yoshimoto;T. Yasukawa;F. Mizutani
中科院分区：
文献类型：
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作者：
Y. Yoshimoto;T. Yasukawa;F. Mizutani
利用顺铂和玻碳电极的简单组合，发展了一种新的检测DNA的电化学技术。顺铂与DNA的络合作用，由于空间位阻的作用，抑制了顺铂的电还原，进而抑制了铂电极上铂对顺铂的电还原催化的质子还原电流。因此，DNA含量是通过电极催化活性的下降来确定的：与顺铂本身相比，测量质子还原电流的电流响应增强，可以提供高灵敏度的顺铂测量。对鲱鱼DNA的检出限为0.1 ng/m L。
A novel electrochemical technique for DNA detection has been developed by a simple combination cisplatin and a glassy carbon electrode. The complexation of cisplatin with DNA caused suppression of the electroreduction of cisplatin owing to the steric hindrance and, subsequently, the electrolytic current for reducing proton that is catalyzed by the platinum deposited on the glassy carbon electrode by the electroreduction of cisplatin. Thus the DNA content was determined from the decrease in the catalytic activity of the electrode: an enhanced current response for measuring the current for reducing proton as compared with that for reducing cisplatin itself could provide the highly-sensitive measurement of cisplatin. The detection limit for herring DNA was as low as 0.1 ng/mL.