Upstream Stimulatory Factors Are Mediators of Ca2+-Responsive Transcription in Neurons
Upstream Stimulatory Factors Are Mediators of Ca2+-Responsive Transcription in Neurons
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DOI：
10.1523/jneurosci.23-07-02572.2003
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发表时间：
2003-04
期刊：
影响因子：
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通讯作者：
Wen G. Chen;A. West;Xu Tao;G. Corfas;M. Szentirmay;M. Sawadogo;C. Vinson;M. Greenberg
中科院分区：
文献类型：
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作者：
Wen G. Chen;A. West;Xu Tao;G. Corfas;M. Szentirmay;M. Sawadogo;C. Vinson;M. Greenberg
为了确定控制 CNS 中活性依赖性基因表达的分子机制，我们对介导 BDNF 启动子 III 活性依赖性转录的因子进行了表征。我们报告了BDNF启动子III内Ca2+响应性E-box元件CaRE2的鉴定，该元件结合上游刺激因子1和2（USF1/2），并表明USF是激活BDNF启动子III的CaRE2依赖性转录所必需的。我们发现USF的转录活性受到神经元中Ca2+激活的信号通路的调节，并且USF在体内与许多神经元活性调节基因的启动子结合。这些结果表明 USF 在调节神经元活动依赖性转录方面具有新功能。
To identify molecular mechanisms that control activity-dependent gene expression in the CNS, we have characterized the factors that mediate activity-dependent transcription ofBDNF promoter III. We report the identification of a Ca2+-responsive E-box element, CaRE2, withinBDNF promoter III that binds upstream stimulatory factors 1 and 2 (USF1/2) and show that USFs are required for the activation of CaRE2-dependent transcription from BDNFpromoter III. We find that the transcriptional activity of the USFs is regulated by Ca2+-activated signaling pathways in neurons and that the USFs bind to the promoters of a number of neuronal activity-regulated genes in vivo. These results suggest a new function for the USFs in the regulation of activity-dependent transcription in neurons.