A novel method for three-dimensional observation of the vascular networks in the whole mouse brain
A novel method for three-dimensional observation of the vascular networks in the whole mouse brain
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DOI：
10.1002/jemt.20522
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发表时间：
2008-01-01
影响因子：
2.5
通讯作者：
Ishikawa, Hiroshi
中科院分区：
文献类型：
--
作者：
Hashimoto, Hisashi;Kusakabe, Moriaki;Ishikawa, Hiroshi
提出了一种适用于小鼠全脑血管网络三维重建的序列图像采集方法。将注入白印度墨水明胶溶液的大脑固定并包埋在含有苏丹黑B的二甲苯和含苏丹黑B的二甲苯中，石蜡块的每个切片表面涂上液体石蜡，连续采集图像。涂上液体石蜡极大地改善了图像的质量。该序列图像没有失真，重建的三维图像没有相邻图像的对准和配准问题。体积再现图像显示了整个大脑中血管的三维分布。在白色印度墨水明胶注入的大脑的体积渲染图像上，没有观察到鬼魂或阴影。由每隔5点获得的系列图像重建的正交面上的z轴分辨率检查没有发现串扰，表明z轴分辨率不大于5gm。正确了解整个大脑中的血管系统对于揭示正常和基因操作小鼠大脑中血管系统的发育以及中风和神经退行性疾病等病理情况下的血管变化是必不可少的。虽然这种方法简单而廉价，但它将提供有关整个大脑血管系统的基本信息。
A novel method for acquiring serial images suitable for three-dimensional reconstruction of vascular networks in the whole brain of mouse was developed. The brain infused with a White India ink-gelatin solution was fixed and embedded in paraffin containing Sudan Black B through xylene also containing Sudan Black B. Each sliced surface of the paraffin block was coated with liquid paraffin and its image was serially acquired. Coating with liquid paraffin extremely improved the quality of the image. The series of serial images was free of distortion and a three-dimensional image was reconstructed without the problem of the alignment and registration of adjacent images. The volume-rendered image indicated three-dimensional distribution of blood vessels in a whole brain. No ghost or shadow was observed on a volume-rendered image of the White India ink-gelatin infused brain. The z-axial resolution examined on the orthogonal sections reconstituted from serial images obtained at an interval of 5 pm showed no cross talk, indicating that the z-axial resolution was no larger than 5 gm. A proper understanding of the vascular system in a whole brain is indispensable to reveal the development of the vascular system in the brain of normal and genetically manipulated mouse and vascular alterations in pathological situation, such as stroke and neurodegenerative disease. Although simple and inexpensive, this method will provide fundamental information on the vascular system in a whole brain.