Quantitative assessment of reactive oxygen sonochemically generated by cavitation bubbles
Quantitative assessment of reactive oxygen sonochemically generated by cavitation bubbles
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DOI：
10.7567/jjap.54.07hf21
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发表时间：
2015-07-01
影响因子：
1.5
通讯作者：
Umemura, Shin-ichiro
中科院分区：
文献类型：
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作者：
Yasuda, Jun;Miyashita, Takuya;Umemura, Shin-ichiro
声空化泡不仅能引起热生物效应，还能引起化学生物效应。当空化气泡破裂并剧烈振荡时，它们会产生活性氧（ROS），导致组织发生不可逆转的变化。一种声敏剂可以促进这种ROS的产生。使用声敏剂的治疗方法称为声动力治疗。红曲是一种体外和体内研究都有报道的超声增敏剂。在声动力处理中，高效产生活性氧是非常重要的。为了有效地产生ROS，提出了一种触发高强度聚焦超声（HIFU）序列。在本研究中，在密封RB溶液的腔室中产生空化气泡，并用高速摄像机捕捉这些空化气泡的行为。用碘化钾（KI）法定量ROS的数量，并与高速相机图像进行比较，以研究触发HIFU序列的有效性。因此，通过该序列可以有效地获得ROS。(C) 2015日本应用物理学会
Acoustic cavitation bubbles can induce not only a thermal bioeffect but also a chemical bioeffect. When cavitation bubbles collapse and oscillate violently, they produce reactive oxygen species (ROS) that cause irreversible changes to the tissue. A sonosensitizer can promote such ROS generation. A treatment method using a sonosensitizer is called sonodynamic treatment. Rose bengal (RB) is one of the sonosensitizers whose in vivo and in vitro studies have been reported. In sonodynamic treatment, it is important to produce ROS at a high efficiency. For the efficient generation of ROS, a triggered high-intensity focused ultrasound (HIFU) sequence has been proposed. In this study, cavitation bubbles were generated in a chamber where RB solution was sealed, and a high-speed camera captured the behavior of these cavitation bubbles. The amount of ROS was also quantified by a potassium iodide (KI) method and compared with high-speed camera pictures to investigate the effectiveness of the triggered HIFU sequence. As a result, ROS could be obtained efficiently by this sequence. (C) 2015 The Japan Society of Applied Physics