Hierarchical Assembly of Nucleic Acid/Coiled-Coil Peptide Nanostructures
Hierarchical Assembly of Nucleic Acid/Coiled-Coil Peptide Nanostructures
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DOI：
10.1021/jacs.9b11158
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发表时间：
2020-01-22
影响因子：
15
通讯作者：
Stephanopoulos, Nicholas
中科院分区：
文献类型：
--
作者：
Buchberger, Alex;Simmons, Chad R.;Stephanopoulos, Nicholas
DNA和多肽是构建自组装材料最常用的两种生物分子，但同时包含这两种成分的杂化纳米结构的例子很少。在这里，我们报道了两个肽的修饰，这两个肽包括具有独特DNA手柄的盘绕异二聚体对，以便将带有互补链的DNA折纸纳米结构连接到微米长的一维阵列中。我们探讨了线圈数目对自组装的影响，并展示了结构的形成通过多条途径：一锅组装，形成二聚体和三聚体，以及两种不同折纸结构的交替共聚物，以及用盘绕-线圈结合物逐步组装纯化的结构。我们的结果表明，两种不同的自组装模式成功地融合在一起，创造了杂化生物材料，有望在未来有一系列潜在的应用。
DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist of hybrid nanostructures that contain both components. Here we report the modification of two peptides that comprise a coiled-coil heterodimer pair with unique DNA handles in order to link DNA origami nanostructures bearing complementary strands into micrometer-long one-dimensional arrays. We probed the effect of number of coils on self-assembly and demonstrated the formation of structures through multiple routes: one-pot assembly, formation of dimers and trimers and an alternating copolymer of two different origami structures, and stepwise assembly of purified structures with coiled-coil conjugates. Our results demonstrate the successful merging of two distinct self-assembly modes to create hybrid bionanomaterials expected to have a range of potential applications in the future.