PHYTOTOXICITY OF DEOXYNIVALENOL TO WHEAT TISSUE WITH REGARD TO IN-VITRO SELECTION FOR FUSARIUM HEAD BLIGHT RESISTANCE
PHYTOTOXICITY OF DEOXYNIVALENOL TO WHEAT TISSUE WITH REGARD TO IN-VITRO SELECTION FOR FUSARIUM HEAD BLIGHT RESISTANCE
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DOI：
10.1016/0168-9452(93)90020-z
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发表时间：
1993-01-01
期刊：
影响因子：
5.2
通讯作者：
SNIJDERS, CHA
中科院分区：
文献类型：
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作者：
BRUINS, MBM;KARSAI, I;SNIJDERS, CHA
四种类型的小麦植物材料，用不同浓度的脱氧雪腐镰刀菌烯醇（DON）和3-乙酰脱氧雪腐镰刀菌烯醇（3-ADON）处理水稻幼苗、胚芽鞘切段、花药愈伤组织和花药胚。DON抑制所有类型的植物材料的生长。根据田间观察到的赤霉病（FHB）抗性水平，大量基因型对4 × 10（-5）M1 DON的幼苗生长反应在耐受和敏感基因型之间没有区别。一般来说，胚芽鞘节段在10（-5）M DON时表现出生长减少。10（-4）M DON的浓度似乎是区分单倍体小麦愈伤组织DON耐受性的最佳浓度。然而，40个愈伤组织克隆的生长分析数据没有显示出任何相关性与已知的FHB抗性水平的原始供体基因型和群体。在含DON的培养基上，胚选择实验中花药衍生胚的再生减少了100倍。在愈伤组织和胚选择实验中平均，绿色植株再生显示在含有毒素的培养基上减少约20倍。
Four types of wheat plant material i.e., seedlings, coleoptile segments, anther-derived callus and anther-derived embryos, were tested at different concentrations of deoxynivalenol (DON) and 3-aceiyldeoxynivalenol (3-ADON). DON inhibited growth of all types of plant material. Seedling growth response to 4 x 10(-5)M 1 DON of a large set of genotypes did not differentiate between tolerant and sensitive genotypes according to observed Fusarium Head Blight (FHB) resistance level in the field. In general, coleoptile segments showed a growth reduction at 10(-5) M DON. A concentration of 10(-4) M DON appeared to be the optimum concentration to differentiate between haploid wheat calli for DON tolerance. However, growth analysis data of 40 callus clones did not show any correlation with the known FHB resistance levels of the original donor genotypes and populations. Regeneration of the anther-derived embryos in the embryo selection experiment was decreased 100-fold on DON-containing medium. Averaged across the callus and embryo selection experiments, green plant regeneration showed a decrease of approximately 20-foId on medium containing toxin.