Sperm DNA damage is associated with assisted reproductive technology pregnancy
Sperm DNA damage is associated with assisted reproductive technology pregnancy
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DOI：
10.1111/j.1365-2605.2007.00803.x
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发表时间：
2008-10-01
影响因子：
--
通讯作者：
Lane, Michelle
中科院分区：
文献类型：
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作者：
Bakos, Hassan W.;Thompson, Jeremy G.;Lane, Michelle
文献表明精子DNA损伤与辅助生殖技术（ART）结果之间存在关联。然而，以前的研究涉及多个胚胎的移植，这使得对结果的解释变得复杂。本研究的目的是确定单胚胎移植后精子DNA损伤水平与受精率、胚胎发育以及妊娠结局之间的关系。对接受体外受精（IVF）（n = 45）和胞质内单精子注射（ICSI）（n = 68）的患者（n = 113）进行了评估，以确定其用于授精的样本中精子DNA损伤水平。采用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记法（TUNEL）检测DNA损伤。采用回归分析评估DNA损伤与预后的关系。总体数据显示，精子DNA损伤与受精率或体外胚胎发育之间没有关联。而IVF授精时，受精率与精子DNA损伤呈显著负相关（p < 0.05）。当ICSI是授精技术时，与植入失败（52.9 +/- 3.9% DNA损伤精子，p < 0.05）相比，低精子DNA损伤与成功妊娠（37.8 +/- 5.7% DNA损伤精子）相关。我们的研究结果表明，精子DNA损伤的TUNEL检测可以提供一个指标，为受精率差的患者和/或那些无法实现怀孕后ART治疗。
The literature suggests an association between sperm DNA damage and assisted reproductive technology (ART) outcomes. However, previous studies involved the transfer of multiple embryos, which has complicated the interpretation of the results. The aim of this study was to determine the relationship between the levels of sperm DNA damage and fertilization rate, embryo development as well as pregnancy outcome, following single embryo transfer. Patients (n = 113) undergoing in vitro fertilization (IVF) (n = 45) and intra-cytoplasmic sperm injection (ICSI) (n = 68) were assessed for their levels of sperm DNA damage in the sample used for insemination. DNA damage was determined using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling (TUNEL). The relationship between DNA damage and outcomes were assessed using regression analysis. Overall data showed no association between sperm DNA damage and fertilization rate, or embryo development in vitro. However, when IVF was the insemination method, there was a significant negative correlation between fertilization rates and sperm DNA damage (p < 0.05). When ICSI was the insemination technique, low sperm DNA damage was associated with successful pregnancy (37.8 +/- 5.7% DNA damaged sperm) compared with failed implantation (52.9 +/- 3.9% DNA damaged sperm, p < 0.05). Our results suggest that sperm DNA damage as measured by the TUNEL assay may provide an indicator for patients with poor fertilization rates and/or those unable to achieve pregnancy following ART treatment.