Early sum frequency generation vibrational spectroscopic studies on peptides and proteins at interfaces
Early sum frequency generation vibrational spectroscopic studies on peptides and proteins at interfaces
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DOI：
10.1116/6.0001859
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发表时间：
2022-05-01
期刊：
影响因子：
2.1
通讯作者：
Chen, Zhan
中科院分区：
文献类型：
--
作者：
Chen, Zhan
本文综述了和频振动光谱技术在研究蛋白质和多肽分子界面性质方面的早期研究成果。SFG研究在C-H伸缩频率区域，以检查蛋白质侧链的行为和在酰胺I频率区域，以调查界面肽/蛋白质的取向和构象。本文还讨论了界面肽/蛋白质的早期手性SFG研究和SFG同位素标记研究。这些早期的SFG研究证明了使用SFG原位阐明肽和蛋白质界面分子结构的可行性，为以后SFG研究界面上的肽和蛋白质奠定了基础。
This paper summarizes the early research results on studying proteins and peptides at interfaces using sum frequency generation (SFG) vibrational spectroscopy. SFG studies in the C-H stretching frequency region to examine the protein side-chain behavior and in the amide I frequency region to investigate the orientation and conformation of interfacial peptides/proteins are presented. The early chiral SFG research and SFG isotope labeling studies on interfacial peptides/proteins are also discussed. These early SFG studies demonstrate the feasibility of using SFG to elucidate interfacial molecular structures of peptides and proteins in situ, which built a foundation for later SFG investigations on peptides and proteins at interfaces.Published under an exclusive license by the AVS.