Acrocentric centromere organization within the chromocenter of the human sperm nucleus
Acrocentric centromere organization within the chromocenter of the human sperm nucleus
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DOI：
10.1002/mrd.1116
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发表时间：
2001-12-01
影响因子：
2.5
通讯作者：
Bartoov, B
中科院分区：
文献类型：
--
作者：
Gurevitch, M;Amiel, A;Bartoov, B
最近有报道称，在人类精子细胞中，着丝粒聚集在细胞核内侧的染色中心。本研究的目的是确定负责rRNA生物合成的五个顶端着丝粒染色体的着丝粒内的组织结构。在精子核轻度解聚以保留尾部结构后，用荧光原位杂交(FISH)标记着丝粒。尾巴被用作细胞核方向的地形学标记。结果表明：(A)5个着丝粒之间的关联性高于随机分布的预期；(B)所有观察到的着丝粒随机分布在染色中心内，约占内核总面积的87%；(C)着丝粒的主要亚群位于占核总面积8.3%的优选区，其峰值在侧轴上为0.6um，在纵轴顶侧为1.0um；以及(D)着丝粒的分散不受核解聚程度的影响。我们的结论是，在人类精子核中，端着丝粒是在染色中心的非定域结构元素内组织的。染色中心的范围可以从整个核的87%的扩展大小到8.3%的优选大小，与核解聚的程度无关。这些发现对与精子细胞功能或早期胚胎发育没有直接关系的核功能(RRNA)具有重要意义。摩尔。是Reprod。戴夫。2001年，60：507-516。(C)2001年Wiley-Liss，Inc.
It has recently been reported that in human sperm cells, the centromeres are clustered in a chromocenter in the interior region of the nucleus. The aim of the present study was to determine the intra-chromocenter organization of the five centromeres of the acrocentric chromosomes responsible for the biosynthesis of rRNA. The acrocentric centromeres were labeled by fluorescence in situ hybridization (FISH) after mild decondensation of the sperm nuclei to preserve the tail structure. The tail was used as a topographical marker for the orientation of the nucleus. The following results were obtained: (a) the association among the five centromeres was higher than expected from random distribution; (b) all the centromeres observed were randomly located within the chromocenter, occupying about 87% of the total area of the internal nucleus; (c) a major subpopulation of centromeres was located in a preferred area occupying 8.3% of the total nuclear area, with a peak 0.6 mum on the lateral axis and 1.0 mum on the apical side of the longitudinal axis; and (d) The dispersion of the centromeres was not influenced by the degree of the nuclear decondensation. We conclude that in human sperm nuclei, the acrocentric centromeres are organized within a nonlocalized structural element in the chromocenter. The chromocenter can range from an expanded size of 87% of the whole nucleus to a preferred size of 8.3% independent of the degree of nuclear decondensation. These findings have important implications for nuclear function (rRNA) that is not directly related to sperm cell function or early embryo development. Mol. Reprod. Dev. 60: 507-516, 2001. (C) 2001 Wiley-Liss, Inc.