In situ pyrimidine dimer determination by laser cytometry.
In situ pyrimidine dimer determination by laser cytometry.
复制标题
通过激光细胞术原位嘧啶二聚体测定。
DOI：
10.1111/j.1751-1097.1989.tb09205.x
复制
发表时间：
1989
影响因子：
3.3
通讯作者：
Trosko,JE
中科院分区：
文献类型：
--
作者：
Mori,T;Wani,AA;D'Ambrosio,SM;Chang,CC;Trosko,JE
By using antiserum against pyrimidine dimers and argon‐laser imaging microspectrofluoro‐metry, we established a new method to determine UV‐induced pyrimidine dimers and their repair in individual human cells. The method was sensitive enough to determine dimers induced by UV dose as low as 2 J/m2. Normal human cells repaired 50 and 60% of total damage within 8 and 24 h after UV irradiation (20 J/m2), butXeroderma pigmentosumcells (complementation group A) were unable to repair any within the same period. Therefore, the method proved to be a quick, easy, sensitive and accurate means to determine pyrimidine dimersin situ.
