Nuclear magnetic resonance studies of intracellular ions in perfused frog heart.
Nuclear magnetic resonance studies of intracellular ions in perfused frog heart.
复制标题
灌注青蛙心脏细胞内离子的核磁共振研究。
DOI：
10.1152/ajpheart.1987.252.6.h1138
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Fossel,ET
中科院分区：
文献类型：
--
作者：
Burstein,D;Fossel,ET
通过核磁共振（NMR）光谱，在灌流的蛙心中观察到细胞内的钠、钾和锂。含有位移试剂三聚磷酸镝的灌注液缓冲液与细胞外共振的数学滤波或预饱和结合使用，以分离细胞内和细胞外钠NMR信号。向灌注液中加入10 μ M哇巴因，用零钾、低钙缓冲液灌注，并用锂替代66%的灌注液钠，导致细胞内钠水平的以下百分比变化（平均值+/- SD）：哇巴因+ 460 +/- 60%（n = 6），零钾+ 300 +/- 30%（n = 3），锂- 51 +/- 6%（n = 3）。当起搏频率从0次/分钟改变到60次/分钟时，观察到细胞内钠增加45%（中间起搏频率成比例变化）。通过NMR测定细胞内钾与钠浓度的比率为2.3，表明用这些NMR方法检测不到大量的细胞内钾。此外，在用含锂灌注液灌注期间观察到细胞内锂。
Intracellular sodium, potassium, and lithium were observed in a perfused frog heart by nuclear magnetic resonance (NMR) spectroscopy. A perfusate buffer containing the shift reagent, dysprosium tripolyphosphate, was used in combination with mathematical filtering or presaturation of the extracellular resonance to separate the intra- and extracellular sodium NMR signals. Addition of 10 microM ouabain to the perfusate, perfusion with a zero potassium, low-calcium buffer, and replacement of 66% of the perfusate sodium with lithium resulted in the following percent changes in the intracellular sodium levels (mean +/- SD): ouabain + 460 +/- 60% (n = 6), zero potassium + 300 +/- 30% (n = 3), and lithium - 51 +/- 6% (n = 3). An increase of 45% in the intracellular sodium was observed when changing the pacing rate from 0 to 60 beats/min (with proportional changes for intermediate pacing rates). The ratio of intracellular potassium to sodium concentration was determined to be 2.3 by NMR, indicating that a substantial amount of the intracellular potassium is undetectable with these NMR methods. In addition, intracellular lithium was observed during perfusion with a lithium-containing perfusate.