Association of the Skn7 and Yap1 Transcription Factors in the Saccharomyces cerevisiae Oxidative Stress Response
Association of the Skn7 and Yap1 Transcription Factors in the Saccharomyces cerevisiae Oxidative Stress Response
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DOI：
10.1128/ec.00328-10
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发表时间：
2011-06-01
期刊：
影响因子：
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通讯作者：
Fassler, J. S.
中科院分区：
文献类型：
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作者：
Mulford, K. E.;Fassler, J. S.
酿酒酵母Sunc7p是一种应激反应转录因子，通过Sln1p组氨酸激酶进行天冬氨酸磷酸化。Snu7p的天冬氨酸磷酸化是激活响应壁应力所需的基因所必需的，但Snu7p也以天冬氨酸磷酸化不依赖的方式激活氧化应激反应基因。氧化应激反应启动子中Yap1p和Snu7p转录因子结合位点的存在以及SKN7和YAP1突变体对氧化应激敏感的表型表明这两个因子共同作用。我们在这里提出了Snu7和YAP1蛋白之间不依赖于DNA的相互作用的证据，涉及Snu7p的接收域和Yap1p的富含半胱氨酸的结构域。当YAP1蛋白在细胞核中积累时，与Yap1p的相互作用可能有助于将SKN7蛋白分配给氧化应激反应启动子。
Saccharomyces cerevisiae Skn7p is a stress response transcription factor that undergoes aspartyl phosphorylation by the Sln1p histidine kinase. Aspartyl phosphorylation of Skn7p is required for activation of genes required in response to wall stress, but Skn7p also activates oxidative stress response genes in an aspartyl phosphorylation-independent manner. The presence of binding sites for the Yap1p and Skn7p transcription factors in oxidative stress response promoters and the oxidative stress-sensitive phenotypes of SKN7 and YAP1 mutants suggest that these two factors work together. We present here evidence for a DNA-independent interaction between the Skn7 and Yap1 proteins that involves the receiver domain of Skn7p and the cysteine-rich domains of Yap1p. The interaction with Yap1p may help partition the Skn7 protein to oxidative stress response promoters when the Yap1 protein accumulates in the nucleus.