NON-SPECIFIC MESANGIAL STAINING WITH ANTIBODIES AGAINST CYTOMEGALOVIRUS IN IMMUNOGLOBULIN-A NEPHROPATHY
NON-SPECIFIC MESANGIAL STAINING WITH ANTIBODIES AGAINST CYTOMEGALOVIRUS IN IMMUNOGLOBULIN-A NEPHROPATHY
复制标题
免疫球蛋白 A 肾病中巨细胞病毒抗体的非特异性系膜染色
DOI：
10.1016/s0140-6736(89)91144-6
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
J. Mestecky
中科院分区：
文献类型：
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作者：
F. Waldo;M. Tomana;W. Britt;B. Julian;J. Mestecky
对12例免疫球蛋白A(IgA)肾病患者的肾小球进行了抗巨细胞病毒(CMV)多克隆抗血清和13种抗CMV单抗混合物的肾小球染色。所有患者肾小球系膜染色均为多克隆抗血清。5例CMV染色与IgA染色部分一致，6例染色分布不一致。单抗仅在1例患者的肾小球系膜染色。血清抗巨细胞病毒抗体IgA和Ig G水平在患者和对照组之间无差异。在免疫印迹分析中，多克隆抗血清在未感染的人成纤维细胞中与三种蛋白发生反应。未感染的成纤维细胞对抗血清的吸收降低了免疫印迹分析的反应性，并消除了六个肾活检样本中的五个肾小球染色。结论：先前报道的抗CMV抗体与IgA肾病患者肾小球的结合可能是污染人类抗原抗体的结果。
The glomerular staining of a commercial polyclonal antiserum against cytomegalovirus (CMV) and of a mixture of 13 monoclonal anti-CMV antibodies in twelve patients with immunoglobulin-A (IgA) nephropathy was examined. The polyclonal antiserum stained the mesangium in all patients. The CMV staining coincided only partially with that of IgA in five patients and the staining distributions were discordant in six. The monoclonal antibodies stained mesangium in only one patient. Serum levels of IgA and IgG anti-CMV antibodies did not differ between patients and controls. In western blot analysis, the polyclonal antiserum reacted with three proteins in uninfected human fibroblasts. Absorption of the antiserum with uninfected fibroblasts reduced the reactivity on western blot analysis and eliminated glomerular staining in five of six renal biopsy samples. It is concluded that the previously reported binding of anti-CMV antibodies to glomeruli from patients with IgA nephropathy may be the result of contaminating antibodies to human antigens.
IgA 肾病患者对口服脊髓灰质炎免疫的全身免疫反应。
DOI：
--
发表时间：
1989
期刊：
Journal of clinical & laboratory immunology
影响因子：
--
作者：
Waldo,FB;Cochran,AM
通讯作者：
Cochran,AM