Carbohydrate metabolism and its possible roles in endodormancy transition in Japanese pear
Carbohydrate metabolism and its possible roles in endodormancy transition in Japanese pear
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DOI：
10.1016/j.scienta.2012.07.009
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发表时间：
2012-09-06
影响因子：
4.3
通讯作者：
Moriguchi, Takaya
中科院分区：
文献类型：
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作者：
Ito, Akiko;Sakamoto, Daisuke;Moriguchi, Takaya
为了阐明碳水化合物在日本梨（Pyrus pyrifolia，'Kosui'）内休眠过程中的作用，研究了梨侧芽和现生枝中碳水化合物浓度及山梨醇和蔗糖代谢相关酶活性的季节变化。“摩苏尔”花芽在12月中下旬解除休眠。在此期间，在地上部淀粉浓度的减少和增加的可溶性糖在花蕾和芽观察，伴随着发病的酸性转化酶和蔗糖合成酶活性的花蕾。这些变化之后，一个显着的积累山梨醇记录在木质部汁液在12月下旬，伴随着相当大的表达山梨醇转运基因（PpSOT 2）在芽。在此之后，在1月中下旬，花蕾表现出增加的活性NAD依赖山梨醇脱氢酶的山梨醇转运蛋白基因（PpSOT 3）的表达增加的同时。因此，我们假设梨花芽调节内休眠的调节系统，根据休眠阶段的甘露醇和蔗糖-catalysts。我们还讨论了山梨醇对内休眠期花芽生长的抑制作用。(c)2012 Elsevier B. V.保留所有权利。
In order to elucidate the possible roles of carbohydrate in Japanese pear (Pyrus pyrifolia, 'Kosui') during endodormancy, seasonal changes of carbohydrate concentrations and the activities of sorbitol- and sucrose-metabolizing enzymes were investigated in the lateral flower buds and current shoots. 'Mosul' flower buds were released from endodormancy between mid- and late December. During this period, a decrease in the shoot starch concentration and an increase of soluble sugars in both the flower buds and shoots were observed, concomitant with the onset of acid invertase and sucrose synthase activity in flower buds. Following these changes, a notable accumulation of sorbitol was recorded in xylem sap in late-December, accompanied by considerable expression of the sorbitol transporter gene (PpSOT2) in shoots. After that, in mid- to late January, flower buds exhibited increased activity of NAD-dependent sorbitol dehythogenase simultaneous to an increase of sorbitol transporter gene (PpSOT3) expression. We, thus, assume that pear flower buds regulate endodormancy by modulating the systems for sorbitoland sucrose-catabolism depending on the dormancy stage. We also discuss the possible inhibitory effect of sorbitol on flower bud growth during endodormancy. (c) 2012 Elsevier B.V. All rights reserved.