Cloning and functional characterization of a fatty acid transport protein (FATP) from the pheromone gland of a lichen moth, Eilema japonica, which secretes an alkenyl sex pheromone.
Cloning and functional characterization of a fatty acid transport protein (FATP) from the pheromone gland of a lichen moth, Eilema japonica, which secretes an alkenyl sex pheromone.
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DOI：
10.1016/j.ibmb.2010.09.008
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发表时间：
2011
影响因子：
3.8
通讯作者：
Shuguang Qian;T. Fujii;K. Ito;R. Nakano;Y. Ishikawa
中科院分区：
文献类型：
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作者：
Shuguang Qian;T. Fujii;K. Ito;R. Nakano;Y. Ishikawa
蛾类的性信息素主要根据末端官能团的存在（I型）或不存在（II型）分为两类。虽然I型性信息素是由信息素腺（PG）中的常见脂肪酸合成的，II型性信息素衍生自推测在卵母细胞中产生的碳氢化合物，并通过血淋巴转运到PG。最近，从家蚕的PG中鉴定出一种脂肪酸转运蛋白（BmFATP），该蛋白产生I型性信息素（bombykol）。BmFATP被证明有助于PG细胞摄取细胞外脂肪酸以合成家蚕酚。为了阐明的存在和功能的FATP在PG的蛾产生II型性信息素，我们探讨了脂肪同源物中表达的PG的地衣蛾，日本爱勒玛，分泌烯基性信息素（II型）。从E. japonica（Ejfatp）主要在PG中表达，并且其表达在羽化后不久上调。EjFATP在大肠杆菌中的功能性表达增强了长链脂肪酸（C18和C20）的摄取，但不是信息素前体烃。据我们所知，这是第一次报告的克隆和功能特性的一个FATP在PG的蛾生产II型性信息素。虽然EjFATP不太可能参与E.日本，Ejfatp的表达模式表明EjFATP在PG中的作用不直接与信息素的生物合成。
Sex pheromones of moths are largely classified into two types based on the presence (Type I) or absence (Type II) of a terminal functional group. While Type-I sex pheromones are synthesized from common fatty acids in the pheromone gland (PG), Type-II sex pheromones are derived from hydrocarbons produced presumably in the oenocytes and transported to the PG via the hemolymph. Recently, a fatty acid transport protein (BmFATP) was identified from the PG of the silkworm Bombyx mori, which produces a Type-I sex pheromone (bombykol). BmFATP was shown to facilitate the uptake of extracellular fatty acids into PG cells for the synthesis of bombykol. To elucidate the presence and function of FATP in the PG of moths that produce Type-II sex pheromones, we explored fatp homologues expressed in the PG of a lichen moth, Eilema japonica, which secretes an alkenyl sex pheromone (Type II). A fatp homologue cloned from E. japonica (Ejfatp) was predominantly expressed in the PG, and its expression is upregulated shortly after eclosion. Functional expression of EjFATP in Escherichia coli enhanced the uptake of long chain fatty acids (C18and C20), but not pheromone precursor hydrocarbons. To the best of our knowledge, this is the first report of the cloning and functional characterization of a FATP in the PG of a moth producing a Type-II sex pheromone. Although EjFATP is not likely to be involved in the uptake of pheromone precursors in E. japonica, the expression pattern of Ejfatp suggests a role for EjFATP in the PG not directly linked to pheromone biosynthesis.