Receptor Binding Activities of Chlorella on Cysteinyl Leukotriene CysLT, Glutamate AMPA, Ion Channels, Purinergic P2Y, Tachykinin NK2 Receptors and Adenosine Transporter
Receptor Binding Activities of Chlorella on Cysteinyl Leukotriene CysLT, Glutamate AMPA, Ion Channels, Purinergic P2Y, Tachykinin NK2 Receptors and Adenosine Transporter
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DOI：
10.1002/ptr.2864
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发表时间：
2010-01-01
影响因子：
7.2
通讯作者：
Shimoda, Minoru
中科院分区：
文献类型：
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作者：
Cheng, Fong-Chi;Feng, Jin-Jye;Shimoda, Minoru
在总共129个体外受体结合测定系统中测试小球藻粉末。结果显示，该粉末以剂量-浓度方式对半胱氨酰白三烯CysLT 2和谷氨酸AMPA具有有效抑制作用，IC 50平均SEM值分别为20 +/- 4.5 μ g/mL和44 +/- 14 μ g/mL。竞争性结合实验中反映的其他中等和较弱活性见于腺苷转运蛋白;钙通道L型，苯并硫氮杂卓;加巴喷丁;红藻氨酸，NMDA-甘氨酸;三磷酸肌醇IP 3;半胱氨酰CysLT（1），LTB 4;嘌呤能P-2 Y;速激肽NK 2; 5-羟色胺5-HT 2B和前列腺素类，血栓烷A（2）。总之，结果表明，在这些受体结合试验中，小球藻粉末的各种抑制作用可以反映其在调节Ca 2+依赖的信号相关靶点中的作用，并且可能与其生物学效应的机制有关。这些结果揭示了可能用于预防或治疗几种病理学的重要的潜在生化活性。根据这些结果，讨论了该产品可能的治疗用途。版权所有（C）2009约翰威利父子有限公司
A Chlorella powder was tested in a total of 129 in vitro receptor binding assay systems. The results showed a potent inhibition of this powder on cysteinyl leukotriene CysLT2, and glutamate AMPA in a dose-concentration manner with IC50 mean SEM values of 20 +/- 4.5 mu g/mL and 44 +/- 14 mu g/mL, respectively. Other moderate and weak activities reflected in competitive binding experiments were seen versus adenosine transporter; calcium channel L-type, benzothiazepine; gabapentin; kainate, NMDA-glycine; inositol trisphosphate IP3; cysteinyl CysLT(1), LTB4; purinergic P-2Y; tachykinin NK2; serotonin 5-HT2B and prostanoid, thromboxane A(2). Together, the results suggest that the various inhibitory effects of Chlorella powder in these receptor binding assays could reflect its actions in modulating Ca2+-dependent signal related targets and might be relevant to the mechanisms of its biological effects. These results reveal important potential biochemical activities that might be exploited for the prevention or treatment of several pathologies. From these results, the possible therapeutic usage of the product is discussed. Copyright (C) 2009 John Wiley & Sons, Ltd.