HIV-1 transforms the monocyte plasma membrane proteome.
HIV-1 transforms the monocyte plasma membrane proteome.
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HIV-1转换单核细胞质膜蛋白质组。
DOI：
10.1016/j.cellimm.2009.03.012
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发表时间：
2009
影响因子：
4.3
通讯作者：
Gendelman, Howard E.
中科院分区：
文献类型：
--
作者：
Kadiu, Irena;Wang, Tong;Schlautman, Joshua D.;Dubrovsky, Larisa;Ciborowski, Pawel;Bukrinsky, Michael;Gendelman, Howard E.
关键词：
How HIV-1 affects the monocyte proteome is incompletely understood. We posit that one functional consequence of virus-exposure to the monocyte is the facilitation of protein transformation from the cytosol to the plasma membrane (PM). To test this, cell surface labeling with CyDye fluorophores followed by 2 dimensional differential in-gel electrophoresis (2D DIGE) and liquid chromatography tandem mass spectrometry (LC/MS-MS) was performed. Fifty three percent of HIV-1 induced proteins were PM associated. These were linked, in large measure, to cellular activation and oxidative stress. They included biliverdin reductase, leukotriene hydrolase A4, heat shock protein 70, and cystatin B. HIV-1 induced PM protein translocation was linked to cathepsin B- and caspase 9, 3-dependent apoptosis. In contrast, PMA-treated monocytes bypassed caspase 3, 9 pathways and lead to cathepsin B-dependent necrosis. These results demonstrate that HIV-1 affects monocyte activation and oxidative stress. These do not affect viral infection dynamics but are linked to stress-induced cell death.
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
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TRUDELL, JR
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4.8
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影响因子：
11.2
作者：
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通讯作者：
Multhoff, G