The chicken 2'-5' oligoadenylate synthetase A inhibits the replication of West Nile virus.
The chicken 2'-5' oligoadenylate synthetase A inhibits the replication of West Nile virus.
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发表时间：
2012-01
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通讯作者：
Hassan T. Tag-El-Din-Hassan-Hassan-T.-Tag-El-Din-Hassan-1398794813;N. Sasaki;Kanako Moritoh;Daisuke Torigoe;A. Maeda;T. Agui
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作者：
Hassan T. Tag-El-Din-Hassan-Hassan-T.-Tag-El-Din-Hassan-1398794813;N. Sasaki;Kanako Moritoh;Daisuke Torigoe;A. Maeda;T. Agui
西尼罗病毒（WNV）是一种病原体，当感染发生在脑部时可引起西尼罗脑炎。先前对小鼠的研究发现，2‘-5’低聚腺苷酸合成酶1b （Oas1b）基因是抵抗西尼罗河病毒感染的决定性因素。此外，已有研究表明，人类OAS1和OASL与对西尼罗河病毒感染的抵抗力有关。西尼罗河病毒通过涉及野生鸟类和蚊子的复杂生命周期在自然界中得以维持。鸟类不仅易感染西尼罗河病毒，而且还充当宿主，从而参与了疾病的传播。先前有报道称，鸡OASL具有低聚腺苷酸合成酶活性。然而，迄今为止，鸡OASL的抗病毒活性尚未确定。在这项研究中，我们通过在哺乳动物细胞中异位表达这种酶，然后用西尼罗河病毒复制子感染这些细胞，来研究鸡OASL可能的抗病毒活性。我们证明鸡OASL对西尼罗河病毒具有抗病毒活性。这是首次有报告显示鸡的OASL与对西尼罗河病毒感染的抵抗力有关。
West Nile virus (WNV) is a pathogen to cause West Nile encephalitis when the infection occurs in the brain. Previous studies in mice identified the 2'-5' oligoadenylate synthetase 1b (Oas1b) gene as a determining factor for resistance to WNV infection. In addition, it has been suggested that human OAS1 and OASL are associated with the resistance to the WNV infection. WNV is maintained in nature through a complex life cycle involving wildbirds and mosquitoes. Birds are not only susceptible to the WNV, but also act as reservoir hosts, thus participating in the spread of the disease. It has previously been reported that chicken OASL possesses the oligoadenylate synthetase activity. However, until now the antiviral activity of chicken OASL has not been determined. In this study, we investigated the putative antiviral activity of chicken OASL by ectopic expression of this enzyme in mammalian cells and then infecting these cells with WNV replicon. We demonstrate that chicken OASL has an antiviral activity against the WNV. This is the first report to show that chicken OASL is associated with the resistance to the WNV infection.