Crystallization of myosin subfragment 1.
Crystallization of myosin subfragment 1.
复制标题
肌球蛋白亚片段1的结晶。
DOI：
10.1073/pnas.81.14.4378
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发表时间：
1984
影响因子：
11.1
通讯作者：
Winkelmann,DA
中科院分区：
文献类型：
--
作者：
Rayment,I;Winkelmann,DA
从鸟类骨骼肌中提取的肌球蛋白亚片段1的晶体已经被可重复地生长。它们将X射线成像到至少4.5 A的分辨率。亚片段1以空间群P2（1）2（1）2（1）结晶，其中a = 107 A，B = 117 A和c = 278 A。X射线衍射照片上的晶胞尺寸和强度分布与晶体学不对称单元中的两个分子一致。电泳分析表明，存在于晶体中的肌球蛋白亚片段1含有95千道尔顿重链片段和必需和调节轻链。
Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract x-rays to at least 4.5-A resolution. The subfragment 1 crystallizes in space group P2(1)2(1)2(1) where a = 107 A, b = 117 A, and c = 278 A. The cell dimensions and intensity distribution on x-ray diffraction photographs are consistent with two molecules in the crystallographic asymmetric unit. Electrophoretic analysis shows that the myosin subfragment 1 present in the crystals contains a 95-kilodalton heavy chain fragment and both the essential and regulatory light chains.