Specific influence of striatal target neurons on the in vitro outgrowth of mesencephalic dopaminergic neurites: a morphological quantitative study
Specific influence of striatal target neurons on the in vitro outgrowth of mesencephalic dopaminergic neurites: a morphological quantitative study
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纹状体靶神经元对中脑多巴胺能神经突体外生长的具体影响：形态学定量研究
DOI：
10.1523/jneurosci.03-11-02292.1983
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发表时间：
1983
影响因子：
12.6
通讯作者：
A. Prochiantz
中科院分区：
文献类型：
--
作者：
S. Denis‐Donini;J. Glowinski;A. Prochiantz
在以前的研究中，我们已经表明，从胚胎小鼠分离的多巴胺能神经元与纹状体靶神经元共培养，在更大程度上摄取和合成多巴胺。我们现在报道纹状体靶细胞也影响多巴胺能神经元的形态。在共培养中，与单独培养中脑神经元或与小脑细胞共培养相比，通过放射自显影观察到的神经炎分支的总长度减少。在与纹状体神经元相同密度的纹状体神经胶质细胞或用纹状体神经胶质细胞或神经元调节的培养基存在下进行的实验表明，纹状体神经元通过特定的神经元-神经元相互作用在体外调节多巴胺能传入生长。
In previous studies, we have shown that dissociated dopaminergic neurons from embryonic mouse in co-culture with striatal target neurons take up and synthesize dopamine to a greater extent. We now report that striatal target cells influence the morphology of dopaminergic neurons as well. In co-culture, the total length of neuritic arborization visualized by autoradiography is reduced when compared to cultures of mesencephalic neurons alone or to co-cultures with cerebellar cells. Experiments performed in the presence of striatal glial cells at the same density as striatal neurons or with media conditioned with striatal glia or neurons suggest that striatal neurons regulate dopaminergic afferent growth in vitro through specific neuro-neuronal interactions.