Periaqueductal gray neurons project to spinally projecting GABAergic neurons in the rostral ventromedial medulla.
Periaqueductal gray neurons project to spinally projecting GABAergic neurons in the rostral ventromedial medulla.
复制标题
周围灰色神经元的围绕灰色神经元在延端腹侧髓质中投射GABA能神经元。
DOI：
10.1016/j.pain.2008.09.009
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发表时间：
2008-11-30
期刊：
影响因子：
7.4
通讯作者：
Aicher SA
中科院分区：
文献类型：
--
作者：
Morgan MM;Whittier KL;Hegarty DM;Aicher SA
吗啡的镇痛作用部分由投射到延髓头端腹内侧（RVM）的导水管周围灰质（PAG）神经元介导。虽然RVM内的神经回路已被描述，RVM神经元和PAG输入和脊髓输出之间的关系是未知的。本研究的目的是确定PAG的GABA能输出神经元是否靶向RVM网状脊髓神经元。免疫细胞化学和共聚焦显微镜显示，PAG神经元广泛投射到RVM神经元投射到脊髓，这些网状脊髓神经元的三分之二似乎是GABA能（含有GAD 67免疫反应性）。大多数（71%）的PAG纤维接触RVM网状脊髓GAD 67免疫反应阳性神经元也含有GAD 67免疫反应。因此，存在从PAG到抑制性RVM网状脊髓神经元的抑制性投射。然而，也有PAG投射到RVM，不包含GAD 67免疫反应性。进行了额外的实验，以确定是否可以通过RVM靶神经元的电生理特性来解释这种投射的异质性。PAG的预测，以电生理定义和aptacellarally填充ON，OFF，和中性细胞在RVM进行了检查。类似于上述报道的模式，GAD 67-和非GAD 67-免疫反应性PAG神经元投射到RVM中的RVM ON、OFF和中性细胞。这些输入包括GAD 67免疫反应性投射到GAD 67免疫反应性ON细胞和非GAD 67投射到GAD 67免疫反应性OFF细胞。这种模式与PAG神经元通过直接激发RVM OFF细胞和抑制ON细胞产生抗伤害感受一致。
The analgesic effects of morphine are mediated, in part, by periaqueductal gray (PAG) neurons that project to the rostral ventromedial medulla (RVM). Although much of the neural circuitry within the RVM has been described, the relationship between RVM neurons and PAG input and spinal output is not known. The objective of this study was to determine whether GABAergic output neurons from the PAG target RVM reticulospinal neurons. Immunocytochemistry and confocal microscopy revealed that PAG neurons project extensively to RVM neurons projecting to the spinal cord, and two-thirds of these reticulospinal neurons appear to be GABAergic (contain GAD67 immunoreactivity). The majority (71%) of PAG fibers that contact RVM reticulospinal GAD67-immunoreactive neurons also contained GAD67 immunoreactivity. Thus, there is an inhibitory projection from PAG to inhibitory RVM reticulospinal neurons. However, there also were PAG projections to the RVM that did not contain GAD67 immunoreactivity. Additional experiments were conducted to determine whether the heterogeneity in this projection can be explained by the electrophysiological character of the RVM target neurons. PAG projections to electrophysiologically defined and juxtacellularly filled ON, OFF, and Neutral cells in the RVM were examined. Similar to the pattern reported above, both GAD67- and non-GAD67-immunoreactive PAG neurons project to RVM ON, OFF, and Neutral cells in the RVM. These inputs include a GAD67-immunoreactive projection to a GAD67-immunoreactive ON cell and non-GAD67 projections to GAD67-immunoreactive OFF cells. This pattern is consistent with PAG neurons producing antinociception by direct excitation of RVM OFF cells and inhibition of ON cells.
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影响因子：
3.3
作者：
Antal, M;Petko, M;StormMathisen, J
通讯作者：
StormMathisen, J
影响因子：
3.3
作者：
Heinricher, MM;Roychowdhury, SM
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作者：
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影响因子：
2.9
作者：
CHENG, ZF;FIELDS, HL;HEINRICHER, MM
通讯作者：
HEINRICHER, MM