Silicon photomultiplier (SPM) detection of low-level bioluminescence for the development of deployable whole-cell biosensors: possibilities and limitations.
Silicon photomultiplier (SPM) detection of low-level bioluminescence for the development of deployable whole-cell biosensors: possibilities and limitations.
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DOI：
10.1016/j.bios.2012.01.008
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发表时间：
2012-03-15
影响因子：
12.6
通讯作者：
Ripp S
中科院分区：
文献类型：
--
作者：
Li H;Lopes N;Moser S;Sayler G;Ripp S
全细胞细菌生物报告器等待具有高灵敏度和强大兼容硬件的小型化光子计数模块，以实现其多功能，现场生物传感器功能的承诺。在这项研究中，我们探索了带有热电冷却器的硅光电倍增管（SPM）的光子计数读出特性，以及检测低水平生物发光信号的可能性。通过模拟LED光源和基因工程荧光假单胞菌生物报告基因5RL产生的生物发光来评估检测性能。与传统的光电倍增管（PMT）相比，结果表明，冷却的光电倍增管对诱导底物浓度（水杨酸盐）在250 ~ 5000 ppb范围内表现出更宽的线性响应。尽管SPM的冷却降低了暗计数率并提高了最小可检测信号，并且数字滤波器的应用提高了信噪比，但在检测极弱光信号方面仍然受到限制，并且仍然是紧凑型光子计数系统设计中的一个挑战。
Whole-cell bacterial bioreporters await miniaturized photon counting modules with high sensitivity and robust compatible hardware to fulfill their promise of versatile, on-site biosensor functionality. In this study, we explore the photon counting readout properties of the silicon photomultiplier (SPM) with a thermoelectric cooler and the possibilities of detecting low-level bioluminescent signals. Detection performance was evaluated through a simulated LED light source and the bioluminescence produced by the genetically engineered Pseudomonas fluorescens bacterial bioreporter 5RL. Compared with the conventional photomultiplier tube (PMT), the results revealed that the cooled SPM exhibits a wider linear response to inducible substrate concentrations (salicylate) ranging from 250 to 5000 ppb. Although cooling of the SPM lowered dark count rates and improved the minimum detectable signal, and the application of a digital filter enhanced the signal-to-noise ratio, the detection of very low light signals is still limited and remains a challenge in the design of compact photon counting systems.