Myosin light-chain kinase contributes to the proliferation and migration of breast cancer cells through cross-talk with activated ERK1/2.
Myosin light-chain kinase contributes to the proliferation and migration of breast cancer cells through cross-talk with activated ERK1/2.
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DOI：
10.1016/j.canlet.2008.05.028
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发表时间：
2008-11
期刊：
影响因子：
9.7
通讯作者：
Xiaolei Zhou;Yi Liu;J. You;Hang Zhang;Xiao-dong Zhang;L. Ye
中科院分区：
文献类型：
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作者：
Xiaolei Zhou;Yi Liu;J. You;Hang Zhang;Xiao-dong Zhang;L. Ye
肌球蛋白轻链激酶（MLCK）在细胞迁移和肿瘤转移中起重要作用。在此，我们使用MLCK的特异性抑制剂ML-7研究了MLCK参与的信号通路在乳腺癌细胞增殖和迁移中的作用。我们的数据表明，20μM ML-7介导的乳腺癌细胞MLCK的减少能够抑制两种平行细胞系的细胞增殖和迁移（MCF-7和LM-MCF/MDA-MB-231）具有不同的转移能力，通过与活化的ERK 1/2相互串扰，其中磷酸化的肌球蛋白轻链（p-MLC）和β-连环蛋白、细胞周期蛋白D1、存活素、和c-Myc作为必需的下游效应物。
Myosin light-chain kinase (MLCK) plays a crucial role in the cell migration and tumor metastasis. Herein, we investigated the signaling pathways involved in MLCK using ML-7, a specific inhibitor of MLCK, in breast cancer cell proliferation and migration. Our data showed that reduction of MLCK in breast cancer cells mediated by 20μM ML-7 was able to depress the cell proliferation and migration using two parallel cell lines (MCF-7 and LM-MCF/MDA-MB-231) with different metastatic abilities through reciprocal cross-talk with activated ERK1/2, in which both phosphorylated myosin light chain (p-MLC) and cascades of β-catenin, cyclin D1, survivin, and c-Myc serve as essential downstream effectors.