Sodium and gating current time shifts resulting from changes in initial conditions.
Sodium and gating current time shifts resulting from changes in initial conditions.
复制标题
钠和门控电流时间因初始条件的变化而产生。
DOI：
10.1085/jgp.81.6.773
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发表时间：
1983-06
影响因子：
3.8
通讯作者：
Bezanilla, F
中科院分区：
文献类型：
--
作者：
Taylor, R E;Bezanilla, F
由于超极化预脉冲，去极化脉冲引起的鱿鱼巨轴突的钠电流和门控电流被延迟，形状几乎没有变化。对于 -140 mV 的预脉冲，延迟几乎完全饱和，约为 45 微秒。在 8 摄氏度时，它们的时间常数为 60 至 180 微秒，预脉冲范围为 -130 至 -150 mV。随着超极化预脉冲幅度的增加，在选通电流期间移动的额外电荷与钠电流的时间延迟的增加之间存在相关性。这些结果强化了这样的结论：门控电流确实与钠通道开放过程密切相关，并提供了有关早期步骤动力学的信息，这些信息隐藏在离子电流测量中。门控和钠电流时移的主要特征以及电荷运动和时移之间的相关性通过钠激活的连续六态模型来复制。
The sodium and gating currents of the squid giant axon elicited by a depolarizing pulse are delayed, with little change in shape, as a result of a hyperpolarizing prepulse. The delays are almost completely saturated, at approximately 45 microseconds, for prepulses to -140 mV. At 8 degrees C they develop with time constants of between 60 and 180 microseconds for prepulses in the -130- to -150-mV range. There is a correlation between the extra charge moved during the gating current and the increase in the time delay of the sodium current as the magnitude of the hyperpolarizing prepulse is increased. These results strengthen the conclusion that the gating current is indeed closely associated with the process of sodium channel opening and provide information concerning the kinetics of the early steps, which are hidden in ionic current measurements. The main features of the gating and sodium current time shifts and the correlation between charge movement and time shifts are duplicated by a sequential six-state model for sodium activation.