Correlation of Synaptic Inputs in the Visual Cortex of Awake, Behaving Mice
Correlation of Synaptic Inputs in the Visual Cortex of Awake, Behaving Mice
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DOI：
10.1016/j.neuron.2018.08.008
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发表时间：
2018-09-19
期刊：
影响因子：
16.2
通讯作者：
Hestrin, Shaul
中科院分区：
文献类型：
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作者：
Arroyo, Sergio;Bennett, Corbett;Hestrin, Shaul
人们对清醒动物神经元相关性的阈下机制知之甚少。在这里，我们在清醒小鼠的视觉皮层（V1）中进行双全细胞记录，以研究上层感觉神经元之间的膜电位（Vm）相关性。我们发现，在安静的清醒状态下，邻近神经元的膜电位显示出较大的相关波动，包括具有不同调谐特性的细胞对。这些波动是由相关的兴奋和紧随其后的抑制（类似于 5 毫秒滞后）驱动的。在视觉刺激期间，低频活动减弱，并且出现连贯的高频振荡，即使对于非偏好的刺激也是如此。这些振荡是由类似滞后的兴奋性和抑制性输入交替产生的。神经元对的去极化时间序列在自发活动和视觉诱发活动期间都是保守的，这表明刻板的激活流程可能会为额外的突触输入产生时间上精确的“机会之窗”。
The subthreshold mechanisms that underlie neuronal correlations in awake animals are poorly understood. Here, we perform dual whole-cell recordings in the visual cortex (V1) of awake mice to investigate membrane potential (Vm) correlations between upper-layer sensory neurons. We find that the membrane potentials of neighboring neurons display large, correlated fluctuations during quiet wakefulness, including pairs of cells with disparate tuning properties. These fluctuations are driven by correlated barrages of excitation followed closely by inhibition (similar to 5-ms lag). During visual stimulation, low-frequency activity is diminished, and coherent high-frequency oscillations appear, even for non-preferred stimuli. These oscillations are generated by alternating excitatory and inhibitory inputs at a similar lag. The temporal sequence of depolarization for pairs of neurons is conserved during both spontaneous- and visually-evoked activity, suggesting a stereotyped flow of activation that may function to produce temporally precise "windows of opportunity'' for additional synaptic inputs.