Macrophages activated as suspension cultures with lymphocyte mediators devoid of antigen become cytotoxic for tumor cells.
Macrophages activated as suspension cultures with lymphocyte mediators devoid of antigen become cytotoxic for tumor cells.
复制标题
悬浮培养物中活化的巨噬细胞不含抗原的淋巴细胞介质对肿瘤细胞具有细胞毒性。
作者：
W. Churchill;Willy F. Piessens;Carol Sulis;John R. David
正常腹膜渗出液细胞（PEC）被激活为悬浮培养物，无论是在富含介质的上清液从邻氯苯甲酰-牛γ-球蛋白（OCB-BGG）刺激的淋巴细胞或在无抗原的Sephadex组分从这些supernatants。孵育24小时后，贴壁细胞组分的PEC，回收的胰蛋白酶从单层细胞，并通过这种技术激活，作为未分级PEC的细胞毒性。淋巴细胞上清液和上清液的无抗原部分诱导相当的巨噬细胞介导的肿瘤细胞毒性。用胰蛋白酶处理活化的巨噬细胞不会改变其细胞毒性能力。
Normal peritoneal exudate cells (PEC) were activated as suspension cultures either in mediator-rich supernatants from o-chlorobenzoyl-bovine gamma-globulin (OCB-BGG) stimulated lymphocytes or in antigen-free Sephadex fractions from these supernants. After 24 hr incubation thration. The adherent cell fractions of PEC, recovered by trypsinization from monolayers and activated by this technique, were as cytotoxic as unfractionated PEC. Lymphocyte supernatants and antigen-free fractions of the supernatants induced comparable macrophage-mediated tumor cytotoxicity. Treatment of activated macrophages with trypsin did not alter their cytotoxic capacity.