Imprinted DNA methylation reprogramming during early mouse embryogenesis at the Gpr1-Zdbf2 locus is linked to long cis-intergenic transcription
Imprinted DNA methylation reprogramming during early mouse embryogenesis at the Gpr1-Zdbf2 locus is linked to long cis-intergenic transcription
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DOI：
10.1016/j.febslet.2012.01.059
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发表时间：
2012-03-23
期刊：
影响因子：
3.5
通讯作者：
Kono, Tomohiro
中科院分区：
文献类型：
--
作者：
Kobayashi, Hisato;Sakurai, Takayuki;Kono, Tomohiro
父本表达的印迹基因Gpr1和Zdbf2形成一个基因簇，其中这两个基因的印迹甲基化区域不同。我们发现了一种新的、父系表达的、长基因间非编码的Zdbf2变体（Zdbf2linc），该变体是在小鼠早期胚胎发育过程中由母系甲基化的Gpr1 DMR转录而来。虽然Gpr1 DMR表现出双等位基因的高甲基化，但在原肠胚形成后很少观察到Zdbf2linc的表达，尽管Zdbf2 DMR的甲基化与原始Zdbf2编码变体的单等位基因转录呈正相关。此外，母体甲基化印记的缺失导致编码和非编码Zdbf2转录本的双等位基因表达以及Zdbf2 DMRs的完全甲基化。在全球范围内，我们的研究结果表明Zdbf2linc在植入后建立继发性表观遗传修饰中的作用。(C) 2012年欧洲生化学会联合会。Elsevier B.V.版权所有。
The paternally-expressed imprinted genes Gpr1 and Zdbf2 form a gene cluster wherein the imprinted-methylated regions of these two genes differ. We identified a novel, paternally expressed, long intergenic non-coding Zdbf2 variant (Zdbf2linc) transcribed from maternally methylated Gpr1 DMR during early embryogenesis in the mouse. While the Gpr1 DMR displayed biallelic hypermethylation, Zdbf2linc expression was rarely observed in the post-gastrulation, despite a positive correlation between the methylation of Zdbf2 DMRs and the mono-allelic transcription of the original Zdbf2 coding variant. Furthermore, lack of the maternal methylation imprint resulted in the biallelic expression of both coding and non-coding Zdbf2 transcripts as well as complete methylation of Zdbf2 DMRs. Globally, our findings suggest the role of Zdbf2linc in the establishment of secondary epigenetic modifications after implantation. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.