Aequorea victoria bioluminescence moves into an exciting new era
Aequorea victoria bioluminescence moves into an exciting new era
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DOI：
10.1016/s0167-7799(98)01184-6
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发表时间：
1998-05-01
影响因子：
17.3
通讯作者：
Badminton, MN
中科院分区：
文献类型：
--
作者：
Kendall, JM;Badminton, MN
生物发光使许多细胞和分子生物学过程的研究发生了革命性的变化，从细胞内信号到基因转录。本文重点介绍了与生物发光有关的两种蛋白质--水母Aequorea Victoria-aequorin和绿色荧光蛋白的化学和生物技术开发。工程重组Aequorin导致了一种新的技术方法来监测细胞器和亚细胞结构域中的钙信号。新一代细胞内钙指示剂已经被生产出来，其中绿色荧光蛋白的工程变体被用来通过分子内荧光-共振-能量转移来探测其离子环境。
Bioluminescence has revolutionized research into many cellular and molecular-biological processes, ranging from intracellular signalling to gene transcription. This article focuses on the chemistry and biotechnological exploitation of the two proteins involved in bioluminescence-of the jellyfish Aequorea victoria - aequorin and green fluorescent protein. Engineered recombinant aequorin has led to a novel technological approach to monitoring calcium signals in organelles and subcellular domains. A new generation of intracellular calcium indicators has been produced in which engineered variants of green fluorescent protein are used to probe their ionic environment using intramolecular fluorescence-resonance-energy transfer.