Maximizing Antibody Production in Suspension-Cultured Mammalian Cells by the Customized Transient Gene Expression Method
Maximizing Antibody Production in Suspension-Cultured Mammalian Cells by the Customized Transient Gene Expression Method
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通过定制的瞬时基因表达方法最大限度地提高悬浮培养哺乳动物细胞的抗体产量
DOI：
10.1271/bbb.120968
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发表时间：
2013-06
影响因子：
1.6
通讯作者：
Xia Ningshao
中科院分区：
文献类型：
--
作者：
You Min;Liu Yanning;Chen Yingwei;Guo Jiyuan;Wu Jun;Fu Yajuan;Shen Rong;Qi Rui;Luo Wenxin;Xia Ningshao
由于蛋白质表达生产率的巨大差异，在本研究中使用定制的瞬时基因表达（TGE）方法来优化三种抗体的瞬时表达。包括宿主细胞、温度、丙戊酸（VPA）处理、各种载体和添加剂在内的若干因素被独立地优化，然后组合以形成针对每种抗体的定制TGE方案。在这种情况下，三种抗体的优化TGE条件彼此不同。与pCDNA3.1表达载体在CHO-S细胞中的TGE相比，8 C11 cAb、37 hAb和10 F7 cAb的表达产量分别提高了16倍、293倍和19倍。对于8 C11 cAb，在不同质粒上共表达L链和H链导致更高的产量。定制的TGE方法是一种替代方法，可以大大提高各种重组蛋白的表达产率。
Due to the great diversity in protein expression productivity, a customized transient gene expression (TGE) method was used in the present study to optimize transient expression of three antibodies. Several factors, including host cells, temperature, valproic acid (VPA) treatment, various vectors, and additives were optimized independently and then combined to form a customized TGE protocol for each antibody. In the event, the optimized TGE conditions for three antibodies were different from each other. Compared with the TGE in CHO-S cells by pCDNA3.1 expression vector, the expression productivities of 8C11 cAb, 37 hAb, and 10F7 cAb showed 16-fold, 293-fold, and 19-fold increases respectively by the customized TGE method. For 8C11 cAb, coexpressing L-chain and H-chain on different plasmids led to higher yields. The customized TGE method is an alternative approach that can greatly improve the expression productivity of a variety of recombinant proteins.
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影响因子：
--
作者：
Wen-xin Luo;Yingwei Chen;Li-feng Li-Li-feng-Li-2107913899;Chenyu Xu;J. Miao;J. Shih;Jun Zhang;N. Xia
通讯作者：
Wen-xin Luo;Yingwei Chen;Li-feng Li-Li-feng-Li-2107913899;Chenyu Xu;J. Miao;J. Shih;Jun Zhang;N. Xia
DOI：
10.1007/bf02634316
发表时间：
1995-09
期刊：
In Vitro Cellular & Developmental Biology - Animal
影响因子：
--
作者：
V. Neumannová;D. Richardson;K. Kriegerbecková;J. Kovar
通讯作者：
V. Neumannová;D. Richardson;K. Kriegerbecková;J. Kovar
影响因子：
100.3
作者：
Beck, Alain;Wurch, Thierry;Corvaia, Nathalie
通讯作者：
Corvaia, Nathalie
影响因子：
2
作者：
González, R;Andrews, BA;Asenjo, JA
通讯作者：
Asenjo, JA
影响因子：
2.2
作者：
Li, Jiandong;Menzel, Christian;Jostock, Thomas
通讯作者：
Jostock, Thomas