Evaluation of the interaction of chlorhexidine and MDP and its effects on the durability of dentin bonding
Evaluation of the interaction of chlorhexidine and MDP and its effects on the durability of dentin bonding
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DOI：
10.1016/j.dental.2020.10.006
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发表时间：
2020-12-01
期刊：
影响因子：
5
通讯作者：
Chen, Chen
中科院分区：
文献类型：
--
作者：
Shen, Jiadi;Xie, Haifeng;Chen, Chen
Objective.本研究旨在探讨氯己定（CHX）与磷酸二氢10-甲基丙烯酰氧基癸酯（MDP）的相互作用及其对牙本质粘接持久性的影响。测试了两种商业粘合剂：不含MDP的粘合剂（Single Bond 2，SB 2）和含MDP的粘合剂（Single Bond Universal，SBU）。将牙齿随机分为6组，并测试微拉伸粘结强度（mu TBS）：Ctr，与SB 2直接粘结; CHX，CHX处理和SB 2; MDP，MDP处理和SB 2; CHX + MDP，组合CHX和MDP处理和SB 2; SBU，与SBU直接粘结; CHX + SBU，CHX处理和SBU。CHX和MDP的潜在相互作用进行了评估，通过测量nanoleakage，原位酶谱，和化学分析表征，通过傅立叶变换红外光谱（FTIR），X射线光电子能谱（XPS）和核磁共振（NMR）。用于mu TBS和纳米渗漏测试的样品首先经受水储存24小时或6个月。Ctr组和CHX组的初始mu TBS值显著低于其他4组（P < 0.05）。蓄水6个月显著削弱了所有组（P < 0.05），其中对照组显示出最低的mu TBS。这一组也比其他五组表现出更明显的纳米渗漏。原位酶谱显示，Ctr组显示最强的荧光，CHX + MDP组显示比CHX或MDP组更强的荧光。FTIR、XPS和NMR表明MDP与羟基磷灰石发生了相互作用。MDP与CHX结合后，NMR未检测到Ca 2+盐峰，具有显著性。单独使用CHX或MDP均可提高牙本质粘结的耐久性。然而，CHX可能会干扰MDP-Ca盐的形成。(C)2020年牙科材料学院。爱思唯尔公司出版All rights reserved.
Objective. This study aimed to evaluate the potential interaction of chlorhexidine (CHX) and 10-methacryloyloxydecyl dihydrogen phosphate (MDP) and its effects on the durability of dentin bonding.Methods. Two commercial adhesives were tested: a MDP-free adhesive (Single Bond 2, SB2) and a MDP-containing adhesive (Single Bond Universal, SBU). Teeth were randomly assigned to six groups and tested for micro-tensile bond strength (mu TBS): Ctr, direct bonding with SB2; CHX, CHX conditioning and SB2; MDP, MDP conditioning and SB2; CHX + MDP, combined CHX and MDP conditioning and SB2; SBU, direct bonding with SBU; CHX + SBU, CHX conditioning and SBU. The potential interaction of CHX and MDP was assessed by measuring nanoleakage, in situ zymography, and chemoanalytic characterization via Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and nuclear magnetic resonance (NMR). Specimens for mu TBS and nanoleakage tests were first subjected to water storage for 24 h or 6 months.Results. The initial mu TBS values of the Ctr and CHX groups were significantly lower than those of the other four groups (P < 0.05). Water storage for 6 months significantly weakened all groups (P < 0.05), with the Ctr group showing the lowest mu TBS. This group also showed more obvious nanoleakage than the other five groups. In situ zymography revealed that the Ctr group showed the strongest fluorescence and that the CHX + MDP group showed greater fluorescence than either CHX or MDP group. FTIR, XPS, and NMR indicated that MDP can interact with hydroxyapatite. NMR detected no Ca2+ salt peak for MDP when it was combined with CHX.Significance. The application of either CHX or MDP alone can improve dentin bond durability. However, CHX may interfere with the formation of MDP-Ca salts. (C) 2020 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.