Intracellular cleavage of notch leads to a heterodimeric receptor on the plasma membrane
Intracellular cleavage of notch leads to a heterodimeric receptor on the plasma membrane
复制标题
DOI：
10.1016/s0092-8674(00)80336-0
复制
发表时间：
1997-07-25
期刊：
影响因子：
64.5
通讯作者：
ArtavanisTsakonas, S
中科院分区：
文献类型：
--
作者：
Blaumueller, CM;Qi, HL;ArtavanisTsakonas, S
先前通过Notch途径进行信号转导的模型已经描述了在细胞表面表达的全长Notch受体。我们目前的证据表明，Notch受体的质膜被切割。这种切割是Notch进化上保守的一般性质，并且在受体向质膜运输时发生在高尔基体网络中。尽管在细胞中可以检测到全长Notch，但它不会到达表面。切割产生C-末端片段N（TM），其似乎在跨膜结构域的N-末端切割，和N-末端片段N-EC，其包含大部分胞外区。我们提供的证据表明，这些片段拴在一起的质膜上的链接，是敏感的还原条件，形成异二聚体受体。
Previous models for signal transduction via the Notch pathway have depicted the full-length Notch receptor expressed at the cell surface. We present evidence demonstrating that the Notch receptor on the plasma membrane is cleaved. This cleavage is an evolutionarily conserved, general property of Notch and occurs in the trans-Golgi network as the receptor traffics toward the plasma membrane. Although full-length Notch is detectable in the cell, it does not reach the surface. Cleavage results in a C-terminal fragment, N(TM), that appears to be cleaved N-terminal to the transmembrane domain, and an N-terminal fragment, N-EC, that contains most of the extracellular region. We provide evidence that these fragments are tethered together on the plasma membrane by a link that is sensitive to reducing conditions, forming a heterodimeric receptor.