Localization of nerve growth factor receptor mRNA in contused rat spinal cord by in situ hybridization.
Localization of nerve growth factor receptor mRNA in contused rat spinal cord by in situ hybridization.
复制标题
通过原位杂交定位挫伤大鼠脊髓中的神经生长因子受体 mRNA。
DOI：
10.1016/0006-8993(91)90298-a
复制
发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Wrathall,JR
中科院分区：
文献类型：
--
作者：
Reynolds,ME;Brunello,N;Mocchetti,I;Wrathall,JR
Northern blot analysis using a probe for the low-affinity nerve growth factor receptor (NGFR) revealed that a mild contusive injury induces the expression of NGFR mRNA in rat spinal cord with a maximal expression at 7 days post-injury. We have now localized this induction using in situ hybridization and found the highest concentration of NGFR mRNA at the lesion epicenter. The location and pattern of autoradiographic grains were compared with that of various cell types at the injury site as determined by immunocytochemical studies. The results suggest that cells associated with blood vessels at the epicenter are induced to express NGFR mRNA at 7 days post-injury.
