Observation on the cycle of the seminiferous epithelium in the Japanese macaque (Macaca fuscata) using semithin sections
Observation on the cycle of the seminiferous epithelium in the Japanese macaque (Macaca fuscata) using semithin sections
复制标题
利用半薄切片观察日本猕猴（Macaca fuscata）生精上皮的周期
作者：
Y. Nagato;T. Enomoto;K. Matsubayashi
日本猕猴的生精上皮细胞周期的阶段进行了研究，使用睾丸固定的甲醛和戊二醛的混合物含有苦味酸和嵌入在甲基丙烯酸酯树脂，Quetol 523 M。用玻璃刀切割1.0-2.0 µm厚的切片，并用过碘酸-希夫（PAS）和苏木精染色。这种树脂块的切片显示了在聚合过程中没有结构变形的细胞细节。此外，与PAS和苏木精的染色亲和力极佳。在染色切片中，根据A型（深色和浅色）精原细胞、B型精原细胞的核形态、减数分裂过程中初级精母细胞的各个发育阶段以及顶体系统的发育，描述了典型的生殖细胞协会。在日本猕猴，两个不同的步骤精子细胞（步骤3和4）不断看到在同一地区的小管上皮在第三阶段。因此，提出了该物种的循环分为10个阶段。其他特征描述的基础上观察生精上皮使用半薄切片。
The stages of the cycle of the seminiferous epithelium in the Japanese macaque are investigated using testes fixed by a mixture of formaldehyde and glutaraldehyde containing picric acid and embedded in a methacrylate resin, Quetol 523M. Sections, 1.0–2.0 µm in thickness, were cut with glass knives and stained with periodic acid-Schiff (PAS) and hematoxylin. Sections from such resin blocks illustrated cellular detail without structural distortion during the polymerization process. Furthermore, staining affinity with PAS and hematoxylin was excellent. In stained sections, typical germ cell associations were described, based on the nuclear morphology of type A (dark and pale) spermatogonium, type B spermatogonium, various developmental stages of primary spermatocytes during meiosis, and the development of the acrosomic system. In the Japanese macaque, two different steps of spermatids (steps 3 and 4) were constantly seen in the same area of the tubular epithelium during stage III. Therefore, a classification into ten stages is proposed for the cycle in this species. Additional characteristics are described based on the observation of the seminiferous epithelium using semithin sections.