Prediction of stroke lesions in stroke-prone spontaneously hypertensive rats by glutathione peroxidase in erythrocytes.
Prediction of stroke lesions in stroke-prone spontaneously hypertensive rats by glutathione peroxidase in erythrocytes.
复制标题
通过红细胞中的谷胱甘肽过氧化物酶预测易发生中风的自发性高血压大鼠的中风病变。
DOI：
10.1271/bbb.59.1459
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
H. Yoshizumi
中科院分区：
文献类型：
--
作者：
T. Murakami;K. Takemori;H. Yoshizumi
通过研究红细胞谷胱甘肽过氧化物酶（GSH-Px）活性与脑卒中病变程度的关系，生物化学方法确定脑卒中易感自发性高血压大鼠（SHRSP）脑卒中发生时间。当shsps血压维持在240 mmHg以上时，GSH-Px活性下降，体重也下降。红细胞GSH-Px活性低于23单位/ml血的SHRSP脑卒中发生率为98% （n = 88/90）。即使GSH-Px活性降至23单位/毫升血液，红细胞压积水平也没有变化。免疫化学滴定表明，持续高血压期间红细胞中GSH-Px活性降低是由于GSH-Px蛋白的减少，而不是酶的失活。当GSH-Px活性降至23单位/ml血液时，将对照饮食改为以鱼为基础的饮食或给予肼治疗，GSH-Px活性恢复，体重增加，寿命延长。由此推断，追踪SHRSP红细胞中GSH-Px活性可作为预测和预后脑卒中病变的指标。
The incipient timing of cerebral strokes in the stroke-prone spontaneously hypertensive rats (SHRSP) was biochemically determined by investigating the relationship between the glutathione peroxidase (GSH-Px) activity in erythrocytes and the extent of stroke lesions. When the blood pressure of SHRSPs was maintained at over 240 mmHg, the GSH-Px activity fell, and the body weight also decreased. In SHRSP whose GSH-Px activity in erythrocytes had dropped below 23 units/ml of blood, the incidence of cerebral strokes was 98% (n = 88/90). The hematocrit level did not change even after the GSH-Px activity had dropped to 23 units/ml of blood. The reduced GSH-Px activity in erythrocytes observed during continued hypertension was found to be due to a decrease in GSH-Px protein, and not to any inactivation of the enzyme, as evident from immunochemical titration. At the moment when the GSH-Px activity had dropped to 23 units/ml of blood, and the control diet was changed to one based on fish or a hydralazine treatment given, the activity recovered, and an increase in body weight and prolongation of the life-span were observed. It was deduced from these findings that tracing the GSH-Px activity in erythrocytes in SHRSP would serve as an indicator for predicting and prognosing stroke lesions.