Gut microbiota induced by dietary GWF® contributes to growth promotion, immune regulation and disease resistance in hybrid sturgeon (Acipenserbaerii x Acipenserschrenckii): Insights from a germ-free zebrafish model
Gut microbiota induced by dietary GWF® contributes to growth promotion, immune regulation and disease resistance in hybrid sturgeon (Acipenserbaerii x Acipenserschrenckii): Insights from a germ-free zebrafish model
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膳食 GWF® 诱导的肠道微生物群有助于杂交鲟（Acipenserbaerii x Acipenserschrenckii）的生长促进、免疫调节和抗病能力：来自无菌斑马鱼模型的见解
DOI：
10.1016/j.aquaculture.2020.734966
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发表时间：
2020
期刊：
影响因子：
4.5
通讯作者：
Youming Zhang
中科院分区：
文献类型：
--
作者：
Xuexiang Wu;Qiang Hao;Tsegay Teame;Qianwen Ding;Hongliang Liu;Chao Ran;Yalin Yang;Liqiu Xia;Shaojun Wei;Zhigang Zhou;Zhen Zhang;Youming Zhang
水生动物的肠道菌群对宿主的多种生理过程有着重要的影响，其中一些肠道细菌具有作为益生菌的潜力。本研究的目的是利用无菌（GF）斑马鱼模型研究生长无忧（GWF®）诱导的杂交鲟鱼（Acipenserbaerii x Acipenserschrenckii）肠道菌群对鲟鱼生长、免疫调节和抗病性的影响。鲟鱼用GWF®补充或基础饮食喂养三周。在实验结束时，将以任一实验饮食喂养的鲟鱼的肠道微生物群转移到GF斑马鱼中三天。检测促生长、炎症和非特异性免疫相关基因的表达。此外，用Veronii气单胞菌Hm 091攻击定殖有任一实验微生物群的斑马鱼两天。结果表明，生长促进相关基因（mTOR、MYoD、肌细胞生成素）和非特异性免疫相关基因（DEFBL-1、溶菌酶、C3 a和抗炎基因TGF-β）的表达在用来自GWF®鲟鱼的微生物群定殖的GF斑马鱼中显著上调（P< .05），而促炎基因IL-1β的表达显著降低（P< .05）。此外，用来自GWF®鲟鱼的微生物群定殖的GF斑马鱼组对A具有显著更高的存活率。veroniHm091（P<0.05）。我们的研究表明，使用无菌斑马鱼模型，GWF®诱导的鲟鱼肠道微生物群在鲟鱼的生长、免疫系统改善和抗病性方面发挥了重要作用。
Gut microbiota of aquatic animals have a crucial impact on several host physiological processes and some of commensal bacteria may have the potential to be used as probiotics. The aim of the present study was to investigate the effects of hybrid sturgeon (Acipenserbaerii x Acipenserschrenckii) gut microbiota induced by growth worry free (GWF®) on the growth, immune regulation and disease resistance in sturgeon using a germ free (GF) zebrafish model. Sturgeons were fed for three weeks with GWF® supplemented or basal diet. At the end of the experiment gut microbiota of sturgeon, fed on either experimental diet were transferred to GF zebrafish for three days. Then the expression of growth promotion, inflammation and non-specific immune related genes were assessed. Moreover, zebrafish colonized with either the experimental microbiota were challenged with Aeromonas veronii Hm091 for two days. Results indicated that the expression of growth promotion related genes (mTOR, MYoD, Myogenin) and non-specific immune related genes (DEFBL-1, lysozyme, C3a, and anti-inflammatory gene TGF-β), was significantly up-regulated in GF zebrafish colonized with microbiota from GWF® sturgeon (P< .05), whereas the expression of pro-inflammatory gene IL-1β was significantly decreased (P< .05). Moreover, group of GF zebrafish colonized with microbiota from GWF® sturgeon had significantly higher survival rate againstA. veroniiHm091 (P< .05). Our study suggests that, the gut microbiota of sturgeon induced by GWF® played a great role in growth, immune system improvement and disease resistance in sturgeon using a germ free zebrafish model.