Integrated Analysis of Glutathione Metabolic Pathway in Pancreatic Cancer.
Integrated Analysis of Glutathione Metabolic Pathway in Pancreatic Cancer.
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胰腺癌中谷胱甘肽代谢途径的综合分析
DOI：
10.3389/fcell.2022.896136
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发表时间：
2022
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
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作者：
代谢酶基因(MES)在各种类型的癌症中起着关键作用。然而，MES在胰腺癌(PC)中的作用尚未得到系统和深入的研究。对PC中MES的全球分析将有助于我们了解PC的进展，并为PC的治疗提供新的见解。在这项研究中，我们系统地分析了来自癌症基因组图谱(TCGA)(n=180+4)和GSE15471(n=36+36)的RNA测序数据，发现PC的代谢途径是混乱的。利用加权基因共表达网络分析(WGCNA)构建了MES的共表达网络模块，确定了两个关键模块。这两个模块都揭示了PC中谷胱甘肽信号通路的紊乱，并与PC的分期相关。值得注意的是，谷胱甘肽过氧化物酶2(GPX2)是谷胱甘肽信号转导途径中的重要基因，是关键模块的中枢基因。免疫微环境成分分析显示PC期与M2巨噬细胞相关，M2巨噬细胞的标志基因与GPX2显著相关。结果表明，GPX2与PC的进展有关，为未来的靶向治疗提供了新的见解。
Metabolic enzyme-genes (MEs) play critical roles in various types of cancers. However, MEs have not been systematically and thoroughly studied in pancreatic cancer (PC). Global analysis of MEs in PC will help us to understand PC progressing and provide new insights into PC therapy. In this study, we systematically analyzed RNA sequencing data from The Cancer Genome Atlas (TCGA) (n = 180 + 4) and GSE15471 (n = 36 + 36) and discovered that metabolic pathways are disordered in PC. Co-expression network modules of MEs were constructed using weighted gene co-expression network analysis (WGCNA), which identified two key modules. Both modules revealed that the glutathione signaling pathway is disordered in PC and correlated with PC stages. Notably, glutathione peroxidase 2 (GPX2), an important gene involved in glutathione signaling pathway, is a hub gene of the key modules. Analysis of immune microenvironment components reveals that PC stage is associated with M2 macrophages, the marker gene of which is significantly correlated with GPX2. The results indicated that GPX2 is associated with PC progression, providing new insights for future targeted therapy.
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WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
9.7
作者：
Gao, Xiaoliang;Jiang, Mingzuo;Fan, Daiming
通讯作者：
Fan, Daiming
影响因子：
14.9
作者：
Xie C;Mao X;Huang J;Ding Y;Wu J;Dong S;Kong L;Gao G;Li CY;Wei L
通讯作者：
Wei L
DOI：
10.3390/molecules15031242
发表时间：
2010-03-03
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Cacciatore I;Cornacchia C;Pinnen F;Mollica A;Di Stefano A
通讯作者：
Di Stefano A
影响因子：
4.4
作者：
Roth, E;Oehler, R;Spittler, A
通讯作者：
Spittler, A