THE SCHIZOSACCHAROMYCES-POMBE CDC14 GENE IS REQUIRED FOR SEPTUM FORMATION AND CAN ALSO INHIBIT NUCLEAR DIVISION
THE SCHIZOSACCHAROMYCES-POMBE CDC14 GENE IS REQUIRED FOR SEPTUM FORMATION AND CAN ALSO INHIBIT NUCLEAR DIVISION
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DOI：
10.1091/mbc.4.5.531
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发表时间：
1993-05-01
影响因子：
3.3
通讯作者：
SIMANIS, V
中科院分区：
文献类型：
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作者：
FANKHAUSER, C;SIMANIS, V
裂殖酵母裂殖酵母cdc14基因的条件性热敏突变导致不能形成隔膜。在没有细胞分裂的情况下，随着生长和核分裂的继续，细胞变得高度细长和多核。本文描述了cdc14基因的克隆及其产物p28cdc14的鉴定。cdc14基因的无效等位基因表明该基因对于隔膜的形成和细胞分裂周期的完成是必不可少的。基因产物p28cdc14的过表达导致细胞周期停滞在有丝分裂前的G2晚期。细胞泄漏过去的块激活p34cdc2激酶，并显示浓缩的染色体，但正常的微管和微丝的重排，与过渡从间期到有丝分裂不发生。突变体中p28cdc14的过表达，其中有丝分裂的时间被改变，表明这些影响可能是有丝分裂抑制剂wee1的上游介导的。这些数据与p28cdc14可能在有丝分裂和隔膜形成的启动中起作用的想法一致，并且通过这样做，成为协调这两个细胞周期事件的机制的一部分。
A conditional heat-sensitive mutation in the cdc14 gene of the fission yeast Schizosaccharomyces pombe results in failure to form a septum. Cells become highly elongated and multinucleate as growth and nuclear division continue in the absence of cell division. This article describes the cloning of the cdc14 gene and the identification of its product, a protein of 240 amino acids, p28cdc14. A null allele of the cdc14 gene shows that the gene is essential for septum formation and completion of the cell-division cycle. Overexpression of the gene product p28cdc14, causes cell-cycle arrest in late G2 before mitosis. Cells leaking past the block activate p34cdc2 kinase and show condensed chromosomes, but the normal rearrangements of the microtubules and microfilaments that are associated with the transition from interphase to mitosis do not occur. Overexpression of p28cdc14 in mutants, in which the timing of mitosis is altered, suggests that these effects may be mediated upstream of the mitotic inhibitor wee1. These data are consistent with the idea that p28cdc14 may play a role in both the initiation of mitosis and septum formation and, by doing so, be part of the mechanism that coordinates these two cell-cycle events.