Mitochondrial membrane potential in 2-cell stage embryos correlates with the success of preimplantation development
Mitochondrial membrane potential in 2-cell stage embryos correlates with the success of preimplantation development
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DOI：
10.1530/rep-13-0288
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发表时间：
2014-05-01
期刊：
影响因子：
3.8
通讯作者：
Kikkawa, Fumitaka
中科院分区：
文献类型：
--
作者：
Komatsu, Kouji;Iwase, Akira;Kikkawa, Fumitaka
超数排卵中的激素刺激诱导雌性小鼠比自发排卵排卵更多的卵母细胞。由于超数排卵的卵母细胞含有许多在自发排卵或未成熟卵母细胞之前通常退化的卵母细胞，因此通过IVF从这些卵母细胞衍生的一些胚胎的发育被阻止。因此，超数排卵的卵母细胞的质量应该不同于自发排卵的卵母细胞。在这项研究中，我们通过检查1-和2-细胞期胚胎来评估超数排卵卵母细胞的质量，其中发育主要取决于卵母细胞中包含的母体mRNA、蛋白质和线粒体，然后我们测量了1-和2-细胞期、体内受精和IVF胚胎的线粒体膜电位（Delta Psi（m））。1-细胞期IVF胚胎的Delta Psi（m）低于体内受精胚胎，但IVF胚胎之间没有差异。在1- 2-细胞期的发育过程中，体内受精胚胎的Delta Psi（m）高度上调，而一些IVF胚胎保持不变。结果，2-细胞期胚胎被分为两组：高和低Delta Psi（m）2-细胞期IVF胚胎。低Δ Psi（m）2-细胞期IVF胚胎的发育倾向于在2-细胞期后停滞。这些结果表明，在1- 2-细胞期Delta Psi（m）的上调在早期植入前胚胎的发育中起重要作用;超数排卵卵母细胞的线粒体存在缺陷，阻碍了其发育。
Hormonal stimulation in superovulation induces female mice to ovulate more oocytes than spontaneous ovulation. Because the superovulated oocytes contain a number of oocytes that normally regress before spontaneous ovulation or immature oocytes, the development of some embryos that derive from these oocytes by IVF is prevented. Therefore, the quality of superovulated oocytes should differ from that of spontaneously ovulated oocytes. In this study, we evaluated the quality of superovulated oocytes, by examining 1- and 2-cell stage embryos, in which the development mainly depends on the maternal mRNA, proteins, and mitochondria that are contained in the oocytes, and we then measured the mitochondrial membrane potential (Delta Psi(m)) of the 1- and 2-cell stage, in vivo-fertilized, and IVF embryos. The Delta Psi(m) of 1- cell stage IVF embryos was lower than that of in vivo-fertilized embryos; however, there was no difference between IVF embryos. During the developmental process from 1- to 2-cell stage, the Delta Psi(m) of in vivo-fertilized embryos was highly upregulated, whereas a number of IVF embryos remained unchanged. As a result, 2-cell stage embryos were divided into two groups: high- and low- Delta Psi(m) 2-cell stage IVF embryos. The development of low- Delta Psi(m) 2-cell stage IVF embryos tended to be arrested after the 2-cell stage. These results indicated that the upregulation of Delta Psi(m) during the 1- to 2-cell stage was important in the development of early preimplantation embryos; there were some defects in the mitochondria of superovulated oocytes, which prevented their development.