A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode.
A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode.
复制标题
DOI：
10.3390/s17010054
复制
发表时间：
2016-12-29
期刊：
影响因子：
--
通讯作者：
Kim HH
中科院分区：
文献类型：
--
作者：
Choi YB;Jeon WY;Kim HH
本研究建立了一种简单、一步检测的同质方法来分析甲苯的主要代谢物马尿酸（HA）。聚乙烯胺咪唑（PVI）在氧化铟锡（ITO）电极上的易固定获得了高灵敏度。采用先前开发的方法，将五氰高铁酸盐与吡啶- n配体配位，并通过静电氰化物桥将氧化活性Fe（II/III）中心与电极上的Ni（II）离子结合。在Ni（II）离子修饰的PVI-ITO （Ni-PVI-ITO）电极上，游离HA和五氰高铁酸-（4-氨基甲基吡啶-马尿酸）（Fe-HA，电子传递介质）与HA抗体竞争结合。采用循环伏安法、差分脉冲伏安法、场发射扫描电镜和x射线光电子能谱法对电极进行了电学和物理化学表征。在低介质浓度下，电信号与HA浓度在0.1µg/mL ~ 1.0 mg/mL之间成正比。同样的方法可以推广到其他小的有机分子。
In this study, a homogeneous method featuring simple, one-step detection was developed to analyze hippuric acid (HA), a major metabolite of toluene. High sensitivity was achieved with the facile immobilization of poly(vinylimidazole) (PVI) on an indium tin oxide (ITO) electrode. Using a previously developed approach, pentacyanoferrate was coordinated with pyridyl-N ligands, and the redox-active Fe(II/III) centers were bound to Ni(II) ions on the electrode via electrostatic cyanide bridges. The detection was accomplished by the competitive binding of free HA and pentacyanoferrate-(4-aminomethylpyridine-hippuric acid) (Fe-HA, the electron transfer mediator) to the HA antibody on the Ni(II) ions-modified PVI-ITO (Ni-PVI-ITO) electrode. The electrical and physicochemical characterization of the electrode was carried out by cyclic voltammetry, differential pulse voltammetry, field emission scanning electron microscopy, and X-ray photoelectron spectroscopy. At low mediator concentrations, the electrical signals were proportional to the HA concentration between 0.1 µg/mL and 1.0 mg/mL. The same method may be extended to other small organic molecules.