Studies on the pathogenesis of atherosclerosis. I. Adhesion and emigration of mononuclear cells in the aorta of hypercholesterolemic rats.
Studies on the pathogenesis of atherosclerosis. I. Adhesion and emigration of mononuclear cells in the aorta of hypercholesterolemic rats.
复制标题
DOI：
--
复制
发表时间：
1983-12
期刊：
影响因子：
--
通讯作者：
I. Joris;T. Zand;J. Nunnari;F. Krolikowski;G. Majno
中科院分区：
文献类型：
--
作者：
I. Joris;T. Zand;J. Nunnari;F. Krolikowski;G. Majno
在饮食诱导的高胆固醇血症大鼠中，在1周内开始出现两种伴随变化，并持续1年：血浆总胆固醇升高和附着在主动脉内膜上的单个核细胞数量增加（高达正常值的50倍）。贴壁细胞约90%为单核细胞，约10%为淋巴细胞。粘连是局灶性的，对主动脉分支的开口有一定的偏爱；随后向内皮下空间迁移。内皮下单核细胞/巨噬细胞逐渐变成泡沫细胞，从而产生显微镜下的“脂肪条纹”。最终形成典型的动脉粥样硬化斑块。提出了四种可能的细胞粘附增强机制。内皮细胞变化轻微；电镜观察内皮细胞表面出现髓磷脂。无论是在AgNO3染色的光镜下还是在超微结构上均未观察到内皮剥落。血小板参与极少。结论：在该模型中，动脉粥样硬化斑块是由单核细胞粘附和迁移引起的；内皮剥落不是其发病的必要步骤。
In rats with diet-induced hypercholesterolemia, two concomitant changes began to occur within 1 week and persisted for 1 year: an increase in total plasma cholesterol and an increase in the number of mononuclear cells adhering to the aortic intima (up to values 50 times normal). Adherent cells were approximately 90% monocytes and approximately 10% lymphocytes. Adhesion was focal, with some preference for ostia of aortic branches; it was followed by migration into the subendothelial space. The subendothelial monocytes/macrophages progressively became foam cells, thus giving rise to microscopic "fatty streaks." Ultimately, typical atherosclerotic plaques were formed. Four possible mechanisms of increased cell adhesion are suggested. Endothelial changes were mild; myelin figures arising from the endothelial surface were seen by electron microscopy. Endothelial denudation was never observed, neither in light-microscopic preparations stained with AgNO3 nor by ultrastructure. Platelet participation was minimal. It is concluded that in this model atherosclerotic plaques are initiated by mononuclear cell adhesion and emigration; endothelial denudation is not a necessary step in their pathogenesis.