G-WIRES - SELF-ASSEMBLY OF A TELOMERIC OLIGONUCLEOTIDE, D(GGGGTTGGGG), INTO LARGE SUPERSTRUCTURES
G-WIRES - SELF-ASSEMBLY OF A TELOMERIC OLIGONUCLEOTIDE, D(GGGGTTGGGG), INTO LARGE SUPERSTRUCTURES
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DOI：
10.1021/bi00201a020
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发表时间：
1994-09-06
期刊：
影响因子：
2.9
通讯作者：
HENDERSON, E
中科院分区：
文献类型：
--
作者：
MARSH, TC;HENDERSON, E
端粒 DNA 寡核苷酸 5'-G(4)T(2)G(4)-3' (Tet 1.5) 自发组装成大型超结构，我们称之为 G 线。 G 线可以通过凝胶电泳解析为梯形图案。 Tet 1.5 的自缔合是非共价的，并表现出 G4-DNA 的特征，G4-DNA 是一种由鸟嘌呤四分体稳定的平行四链结构。 G 线的形成取决于 Na+ 和/或 K+ 的存在，并且一旦形成，G 线就不会变性。这里描述的结果扩展了我们对富含 G 的核酸的结构潜力的理解，并且可以深入了解富含 G 的序列的可能作用以及它们在生物系统中可以形成的新结构。
The telomeric DNA oligonucleotide 5'-G(4)T(2)G(4)-3' (Tet 1.5) spontaneously assembles into large superstructures we have termed G-wires. G-wires can be resolved by gel electrophoresis as a ladder pattern. The self-association of Tet 1.5 is noncovalent and exhibits characteristics of G4-DNA, a parallel four-stranded structure stabilized by guanine tetrads. Formation of G-wires is dependent upon the presence of Na+ and/or K+, and once formed, G-wires are resistant to denaturation. The results described here extend our understanding of the structural potential of G-rich nucleic acids and may provide insight into the possible roles of G-rich sequences and the novel structures they can form in biological systems.