Lymphoid potential, probed before circulation in mouse, is restricted to caudal intraembryonic splanchnopleura
Lymphoid potential, probed before circulation in mouse, is restricted to caudal intraembryonic splanchnopleura
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DOI：
10.1016/s0092-8674(00)80166-x
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发表时间：
1996-09-20
期刊：
影响因子：
64.5
通讯作者：
Godin, I
中科院分区：
文献类型：
--
作者：
Cumano, A;DieterlenLievre, F;Godin, I
造血干细胞在哺乳动物胚胎中的出现尚未得到明确的分配。以前，我们在移植后8.5天（dpc）开始在背主动脉周围区域（主动脉旁内脏胸膜）检测到多能造血前体。然而，由于循环已经建立，造血前体是原位产生还是血液输送仍不清楚。通过增加一个器官型的步骤，我们以前的培养系统，我们现在检测淋巴细胞和多能骨髓前体细胞的胚内内脏胸膜早在7.5 dpc。在相同的条件下，来自相同胚胎的卵黄囊不能产生淋巴后代，并且具有降低的髓样分化和维持的潜力。因此，如果在循环前分离，卵黄囊不会产生多能前体，因此不会促进小鼠的最终造血。
Emergence of hemopoietic stem cells in the mammalian embryo has yet to be definitively allocated. Previously, we detected multipotent hemopoietic precursors in the region surrounding the dorsal aorta (paraaortic splanchnopleura) beginning at 8.5 days postcoitum (dpc). However, as circulation is already established, it remained unclear whether hemopoietic precursors arise in situ or are blood-delivered. By adding an organotypic step to our former culture system, we now detect lymphocyte and multipotent myeloid precursors from the intraembryonic splanchnopleura as early as 7.5 dpc. Under identical conditions, yolk sacs from the same embryos are unable to generate lymphoid progeny and have a reduced potential for myeloid differentiation and maintenance. Thus, if isolated before circulation, the yolk sac does not produce multipotent precursors and therefore does not contribute to definitive hemopoiesis in the mouse.