Genomic structure and cloned cDNAs predict that four variants in the kinase domain of serine/threonine kinase receptors arise by alternative splicing and poly(A) addition.
Genomic structure and cloned cDNAs predict that four variants in the kinase domain of serine/threonine kinase receptors arise by alternative splicing and poly(A) addition.
复制标题
DOI：
10.1073/pnas.91.17.7957
复制
发表时间：
1994-08
影响因子：
11.1
通讯作者：
J. Xu;K. Matsuzaki;K. Mckeehan;F. Wang;M. Kan;W. Mckeehan
中科院分区：
文献类型：
--
作者：
J. Xu;K. Matsuzaki;K. Mckeehan;F. Wang;M. Kan;W. Mckeehan
I型和II型丝氨酸/苏氨酸激酶受体单体的异二聚体构成了转化生长因子β家族配体的活性受体复合体。在这里，我们证明了广泛表达的I型丝氨酸/苏氨酸激酶受体胞内区的编码序列的基因组组织类似于激活素II型受体基因。基因组结构和cDNA克隆表明，在编码外显子-内含子连接的三个羧基末端的交替外显子上添加多聚(A)可能是I型和II型受体基因的共同特征。预测的产物是在激酶亚域VII、IX和X截断的单体，这些亚域的激酶活性和潜在的丝氨酸、苏氨酸和酪氨酸磷酸化位点不同。这些结果表明，在转化生长因子β配体家族中，影响I型和II型受体单体的信号转导细胞内激酶域的变异体的组合可能增加了该家族中单个配体的生物效应的异质性。
Heterodimers of types I and II serine/threonine kinase receptor monomers compose the active receptor complex for ligands of the transforming growth factor beta family. Here we show that the genomic organization of coding sequences for the intracellular domain of a widely expressed type I serine/threonine kinase receptor is similar to that of the activin type II receptor gene. The genomic structure and cDNA clones indicate that poly(A) addition to alternative exons at each of three carboxyl-terminal coding exon-intron junctions may be a common feature of both type I and II receptor genes. The predicted products are monomers truncated at kinase subdomains VII, IX, and X which vary in kinase activity and potential serine, threonine, and tyrosine phosphorylation sites. These results suggest that combinations of variants that affect the signal-transducing intracellular kinase domain of both type I and II receptor monomers within the transforming growth factor beta ligand family may add to the heterogeneity of biological effects of individual ligands in the family.